Bresler S E, Kazbekov E N, Sukhodolova A T, Shadrin V N
Biokhimiia. 1979 Apr;44(4):741-7.
The reaction of terminal oxidation of the substrate (catechol) by molecular oxygen catalyzed by o-diphenoloxidase (o-diphenol: oxygen oxydoreductase; EC 1.10.3.1) is found to occur via a free radical mechanism. The copper of the active center changes its valency during the reaction. The spectra of substrate radicals and of the Cu2+ ions were registered by means of a high sensitivity ESR-spectrometer and their concentrations were determined.
邻二酚氧化酶（邻二酚：氧氧化还原酶；EC 1.10.3.1）催化底物（儿茶酚）与分子氧的末端氧化反应，发现其通过自由基机制发生。活性中心的铜在反应过程中改变其化合价。借助高灵敏度电子自旋共振光谱仪记录底物自由基和Cu2+离子的光谱，并测定其浓度。
