State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
J Phys Chem B. 2011 Nov 24;115(46):13695-700. doi: 10.1021/jp206497u. Epub 2011 Oct 27.
Numbers of reports have demonstrated the merits of confining enzyme in mesoporous supports, but have ignored the limitations to the application for biocatalytic transformation of a macrosubstrate. So in this work a strategy based on pH-triggered release and recovery of proteins has been presented to overcome this restriction. By modulation of the pH value, the encapsulated lysozyme was released from the mesopores to act on the macrosubstrate, displaying full bacteriolytic activity, and then was basically readsorbed by another pH modulation. The protein release-recovery is directly confirmed by confocal laser scanning microscopy (CLSM) and X-ray diffraction (XRD) observations. In virtue of substrate-enhanced desorption and privileged readsorption of lysozyme, high bioactivity and good reusability have been achieved.
已有大量报告证明了将酶限制在介孔载体中的优点，但忽略了在大分子底物的生物催化转化中应用的局限性。因此，在这项工作中，提出了一种基于 pH 触发释放和回收蛋白质的策略，以克服这一限制。通过调节 pH 值，包封的溶菌酶从介孔中释放出来作用于大分子底物，显示出完全的溶菌活性，然后通过另一个 pH 调节基本上被再次吸附。通过共聚焦激光扫描显微镜 (CLSM) 和 X 射线衍射 (XRD) 观察直接证实了蛋白质的释放-回收。由于溶菌酶的底物增强解吸和优先再吸附，实现了高生物活性和良好的可重复使用性。
