Xue Weichang, Bandyopadhyay Amit, Bose Susmita
W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA.
Acta Biomater. 2009 Jun;5(5):1686-96. doi: 10.1016/j.actbio.2009.01.012. Epub 2009 Jan 22.
The purpose of this study is to synthesize mesoporous calcium silicate (CS or wollastonite, CaSiO(3)) and evaluate its possible application in protein/drug delivery. First, calcium silicate was synthesized by wet chemical method and then mesoporosity was created by acid modification of the synthesized CS particle using hydrochloric acid at pH 7, 4.5, and 0.5. The results showed that a hydrated silica gel with abundant Si-OH functional group formed on the surface of calcium silicate due to acid modification. This surface layer had mesoporous structure, with pore diameter between 4 and 5 nm. BET specific average surface area increased to 221, 333, and 356 m(2) g(-1) due to acid modification at pH 7, 4.5, and 0.5, respectively, whereas the surface area for unmodified CS particles was 65 m(2) g(-1). Protein adsorption studies indicated that mesoporous CS has higher ability to adsorb bovine serum albumin and lysozyme compared to unmodified particles. The release kinetics showed that proteins on mesoporous CS released sequentially over one week, whereas the proteins on unmodified particle followed burst release kinetics within a few hours. Human osteoblast cell-material interaction study showed that these materials were biocompatible and promoted excellent bone cell proliferation. In summary, this work has demonstrated the potential to produce mesoporous CS as a carrier for protein/drug delivery for bone regeneration and other biomedical applications.
本研究的目的是合成介孔硅酸钙（CS或硅灰石，CaSiO₃）并评估其在蛋白质/药物递送中的潜在应用。首先，通过湿化学方法合成硅酸钙，然后使用盐酸在pH值为7、4.5和0.5的条件下对合成的CS颗粒进行酸改性以产生介孔结构。结果表明，由于酸改性，在硅酸钙表面形成了具有丰富Si-OH官能团的水合硅胶。该表面层具有介孔结构，孔径在4至5纳米之间。由于在pH值为7、4.5和0.5时进行酸改性，BET比平均表面积分别增加到221、333和356 m² g⁻¹，而未改性的CS颗粒的表面积为65 m² g⁻¹。蛋白质吸附研究表明，与未改性颗粒相比，介孔CS具有更高的吸附牛血清白蛋白和溶菌酶的能力。释放动力学表明，介孔CS上的蛋白质在一周内依次释放，而未改性颗粒上的蛋白质在几小时内遵循突发释放动力学。人成骨细胞与材料相互作用研究表明，这些材料具有生物相容性并促进了优异的骨细胞增殖。总之，这项工作证明了生产介孔CS作为用于骨再生和其他生物医学应用的蛋白质/药物递送载体的潜力。
