Department of Ophthalmology, "Lozano Blesa" Clinical University Hospital, Zaragoza, Spain.
Am J Ophthalmol. 2012 Mar;153(3):399-404. doi: 10.1016/j.ajo.2011.07.025. Epub 2011 Oct 11.
To evaluate in vitro the amoebicidal effects of riboflavin and ultraviolet A (UVA) collagen cross-linking.
Experimental study, laboratory investigation.
Two different strains of Acanthamoeba species were tested identically. Four treatment groups were considered: group 1 consisted of 0.1% riboflavin and 30-minute UVA irradiation; group 2 consisted of 0.1% riboflavin and 60-minute UVA irradiation; group 3 consisted of no riboflavin and no UVA exposure; group 4 consisted of 0.1% riboflavin and no UVA exposure. The application of UVA was performed under the parameters used for in vivo corneal collagen cross-linking.
In all cases, cysts and trophozoites were detected 24 hours after treatment at a radial distance from the center of the seeding point more than 5 mm, indicating that the amoebae were viable. All treated and untreated groups of amoebae from the 2 strains exhibited growth (radii of 14 to 15 mm in groups 1, 3, and 4; radius of 12 mm in group 2). The final morphologic features of the 2 strains of trophozoites that received treatment were similar to those of the initial seeding group and the untreated control group.
The results obtained in our study show that a single dose (30 or 60 minutes) of cross-linking cannot achieve eradication in the 2 different Acanthamoeba strains examined. However, in vitro results do not always indicate in vivo efficacy, so future studies should test the validity of this treatment for Acanthamoeba keratitis.
评估核黄素和紫外线 A（UVA）胶原交联的体外杀阿米巴效果。
实验研究，实验室研究。
两种不同的棘阿米巴属菌株以相同的方式进行测试。考虑了四个治疗组：第 1 组包含 0.1%核黄素和 30 分钟的 UVA 照射；第 2 组包含 0.1%核黄素和 60 分钟的 UVA 照射；第 3 组既没有核黄素也没有 UVA 暴露；第 4 组包含 0.1%核黄素且没有 UVA 暴露。UVA 的应用是在用于体内角膜胶原交联的参数下进行的。
在所有情况下，在处理后 24 小时，在距接种点中心的径向距离超过 5 毫米的地方都检测到了囊肿和滋养体，这表明阿米巴原虫是存活的。来自两种菌株的所有经处理和未经处理的阿米巴虫组都表现出生长（第 1、3 和 4 组的半径为 14 至 15 毫米；第 2 组的半径为 12 毫米）。接受治疗的两种棘阿米巴属滋养体的最终形态特征与初始接种组和未经处理的对照组相似。
我们的研究结果表明，单次剂量（30 或 60 分钟）的交联不能在两种不同的棘阿米巴属菌株中达到根除效果。然而，体外结果并不总是表明体内疗效，因此未来的研究应该测试这种治疗棘阿米巴角膜炎的有效性。
