Institute for Hygiene and Microbiology, University of Würzburg, Josef-Schneider-Str. 2, Building E2, 97080 Würzburg, Germany.
Int J Med Microbiol. 2011 Dec;301(8):659-62. doi: 10.1016/j.ijmm.2011.09.001. Epub 2011 Oct 12.
Neisseria meningitidis causes life-threatening disease in infants, toddlers, and adolescents. Besides representative case notification, public health management of the disease requires bacterial typing information. European reference laboratories and state epidemiologists in collaboration with European institutions have driven forward the harmonization of typing by rigorously adopting DNA sequence typing and using common reference databases. External quality assessment has been provided by supranational networks, i.e. EU-IBIS and IBD-Labnet. The recent development of novel protein-based vaccines targeting serogroup B strains highlights the necessity to complement standard typing schemes by specific vaccine antigen typing including antigen expression analysis. Although not yet feasible for routine application on hundreds of strains, novel database structures have been developed to accommodate deep sequencing data.
脑膜炎奈瑟菌可引起婴幼儿和青少年的生命威胁性疾病。除了有代表性的病例报告外，疾病的公共卫生管理还需要细菌分型信息。欧洲参考实验室和国家流行病学家与欧洲机构合作，通过严格采用 DNA 序列分型并使用共同的参考数据库，推动了分型的协调统一。跨国网络，即欧盟-IBIS 和 IBD-Labnet，提供了外部质量评估。最近针对 B 群菌株的新型基于蛋白的疫苗的发展突出表明，有必要通过包括抗原表达分析在内的特定疫苗抗原分型来补充标准分型方案。尽管尚未可用于数百种菌株的常规应用，但已经开发了新的数据库结构来容纳深度测序数据。
