Department of Clinical Biochemistry, University Hospital South Manchester NHS Foundation Trust, Wythenshawe Hospital, Wythenshawe, Manchester, UK.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Feb 1;883-884:33-49. doi: 10.1016/j.jchromb.2011.09.041. Epub 2011 Sep 29.
LC-MS/MS is an increasingly important tool in therapeutic drug monitoring as it offers increased sensitivity and specificity compared to other methods, and may be the only viable method for quantifying drugs without natural chromophores or fluorophores. The choice of sample preparation method, column technology, internal standard and mass spectrometric conditions is important to ensure accurate drug measurement and to avoid interference from matrix effects and drug metabolites. LC-MS/MS is a more involved technique than automated immunoassays, but technological advances such as the development of pipetting robots and online solid phase extraction mean that LC-MS/MS is becoming an attractive and convenient method for therapeutic drug monitoring in clinical laboratories.
LC-MS/MS 是治疗药物监测中越来越重要的工具，因为与其他方法相比，它具有更高的灵敏度和特异性，并且可能是唯一可行的方法，可用于定量分析没有天然发色团或荧光团的药物。选择样品制备方法、色谱柱技术、内标和质谱条件对于确保药物的准确测量以及避免基质效应和药物代谢物的干扰很重要。LC-MS/MS 比自动化免疫分析更复杂，但移液机器人和在线固相萃取等技术的进步意味着 LC-MS/MS 正成为临床实验室治疗药物监测的一种有吸引力和方便的方法。
