Sallustio Benedetta C
Department of Clinical Pharmacology, Basil Hetzel Institute, The Queen Elizabeth Hospital, Adelaide, South Australia 5011, Australia. benedetta.sallustio@ health.sa.gov.au
Bioanalysis. 2010 Jun;2(6):1141-53. doi: 10.4155/bio.10.58.
Due to their narrow therapeutic indices and highly variable pharmacokinetics, therapeutic drug monitoring is necessary to individualize immunosuppressant dosage following organ transplantation. Until recently, monitoring was performed primarily using immunoassays, however, there is an increasing shift to HPLC coupled with MS/MS, due to its greater sensitivity and specificity. Online sample clean-up with either a single analytical column or with 2D chromatography significantly reduces manual handling and is essential to minimize matrix effects and maximize specificity and, coupled with rapid chromatography, allows the simultaneous analysis of the major immunosuppressants, with rapid sample throughput. Thus, LC-MS/MS is an attractive and versatile technique that facilitates rapid development of analytical methods, including new immunosuppressants as they become approved for clinical use.
由于免疫抑制剂的治疗指数狭窄且药代动力学高度可变，因此在器官移植后进行治疗药物监测以实现免疫抑制剂剂量个体化是必要的。直到最近，监测主要使用免疫测定法进行，然而，由于其更高的灵敏度和特异性，正日益转向采用与串联质谱联用的高效液相色谱法。使用单一分析柱或二维色谱进行在线样品净化可显著减少人工操作，对于最大限度地减少基质效应、最大化特异性至关重要，并且与快速色谱相结合，能够同时分析主要的免疫抑制剂，实现快速的样品通量。因此，液相色谱 - 串联质谱是一种有吸引力且用途广泛的技术，有助于快速开发分析方法，包括新的免疫抑制剂在获批临床使用时。
