School of Electrical Engineering and Computer Science, Seoul National University, Seoul, Korea.
Neuromodulation. 2010 Oct;13(4):299-304; discussion 305. doi: 10.1111/j.1525-1403.2010.00296.x.
This paper examined the efficacy of an implantable electrical stimulator in rats for the functional regeneration of peripheral nerves.
The implantable electrical stimulator was fabricated on a polyimide-based conduit with an integrated electrode, a stimulation chip, and a battery; 3 mg/mL of collagen gel was coated onto the conduit surface and electrical stimulation (20 µ A, 100 µ s, and 100 Hz biphasic current) was continuously applied between the nerve stumps for four weeks. The stimulator was tested on a severed sciatic nerve with a 7-mm gap in rats. The effects of both the electrical stimulation and the collagen application were examined.
Functionality was evaluated through walk track assessments and by recording the action potential of the regenerated nerve. Immunohistochemical staining of the regenerated nerve was done using peripheral myelin protein 22.
The results suggest that the functional recovery of a severed peripheral nerve by the proposed implantable electrical stimulator was achieved through electrical current stimulation along the use of a collagen coating on the conduit surface.
本文研究了一种可植入式电刺激器在大鼠周围神经功能再生中的疗效。
可植入式电刺激器是在聚酰亚胺基导管上制作的，带有集成电极、刺激芯片和电池；将 3mg/mL 的胶原凝胶涂覆在导管表面，并在神经断端之间持续施加电刺激（20µA、100µs 和 100Hz 双相电流），持续四周。在大鼠 7mm 间隙的坐骨神经切断模型上对刺激器进行了测试。同时考察了电刺激和胶原应用的效果。
通过行走轨迹评估和记录再生神经的动作电位来评估功能。使用外周髓鞘蛋白 22 对再生神经进行免疫组织化学染色。
研究结果表明，所提出的可植入式电刺激器通过在导管表面使用胶原涂层进行电流刺激，实现了对切断的周围神经的功能恢复。
