睾酮或胰岛素治疗对去势或链脲佐菌素诱导的糖尿病大鼠输精管收缩反应的影响。

The effects of testosterone or insulin treatment on contractile responses of the rat vas deferens following castration or streptozotocin-induced diabetes mellitus.

Longhurst P A

Division of Urology, Hospital of the University of Pennsylvania, Philadelphia 19104-4283.

Gen Pharmacol. 1990;21(4):427-34. doi: 10.1016/0306-3623(90)90693-g.

Abstract

Castration and streptozotocin-induced diabetes produce significant decreases in serum testosterone levels accompanied by decreased vas deferens weights, a decreased responsiveness to nerve stimulation, and altered contractile responses to carbachol and phenylephrine. 2. Treatment of castrated rats with testosterone for 8 weeks prevented the decreased vas deferens weights and contractile changes associated with castration. 3. Treatment of diabetic rats with testosterone for 8 weeks prevented the decreased vas deferens weights and the supersensitivity to contractile agonists associated with diabetes. Testosterone treatment only partially prevented the decreased response to nerve stimulation. 4. Treatment of diabetic rats with testosterone plus insulin for 8 weeks prevented the decreased vas deferens weights and decreased the sensitivity to carbachol and phenylephrine compared to controls. Testosterone plus insulin treatment prevented the decreased response to nerve stimulation. 5. There were no differences in the IC50 values for nitrendipine among any of the groups studied, suggesting that the contractile changes observed in vasa deferentia following castration or diabetes are not the result of changes in calcium movements. 6. The results suggest that decreased testosterone levels are at least partially responsible for the changes in contractility of the vas deferens of streptozotocin-diabetic rats.

摘要

去势和链脲佐菌素诱导的糖尿病会导致血清睾酮水平显著降低，同时伴有输精管重量减轻、对神经刺激的反应性降低以及对卡巴胆碱和去氧肾上腺素的收缩反应改变。2. 用睾酮治疗去势大鼠8周可防止与去势相关的输精管重量减轻和收缩变化。3. 用睾酮治疗糖尿病大鼠8周可防止与糖尿病相关的输精管重量减轻以及对收缩激动剂的超敏反应。睾酮治疗只能部分防止对神经刺激的反应降低。4. 用睾酮加胰岛素治疗糖尿病大鼠8周可防止输精管重量减轻，且与对照组相比，降低了对卡巴胆碱和去氧肾上腺素的敏感性。睾酮加胰岛素治疗可防止对神经刺激的反应降低。5. 在所研究的任何组中，尼群地平的半数抑制浓度（IC50）值均无差异，这表明去势或糖尿病后输精管中观察到的收缩变化不是钙转运变化的结果。6. 结果表明，睾酮水平降低至少部分导致了链脲佐菌素诱导的糖尿病大鼠输精管收缩性的变化。