Pentadiplandra brazzeana Baillon 中硫代葡萄糖苷的特征和定量分析。
Profile and quantification of glucosinolates in Pentadiplandra brazzeana Baillon.
机构信息
Agricultural Research Council - Industrial Crop Research Centre, Via di Corticella 133, 40128 Bologna, Italy.
出版信息
Phytochemistry. 2012 Jan;73(1):51-6. doi: 10.1016/j.phytochem.2011.09.006. Epub 2011 Oct 10.
Glucosinolates (GLs) present in root, seed, and leaf extracts of Pentadiplandra brazzeana Baillon were characterized and quantified according to the ISO 9167-1 method based on the HPLC analysis of desulfo-GLs. The analyses were complemented by GC-MS analyses of the isothiocyanates (ITCs) generated from GL degradation by myrosinase. Glucotropaeolin (1a), glucolimnanthin (2a), and glucoaubrietin (3a) were shown to be present in the root extract, whereas the seed mainly contained 3a. 3,4-Dimethoxybenzyl GL (4a), glucobrassicin (5a) and traces of 1a were detected in the leaf extract. The products were fully characterized as their desulfo-counterparts by spectroscopic techniques.
根据 HPLC 分析脱磺基 GL 的方法，按照 ISO 9167-1 标准，对 Pentadiplandra brazzeana Baillon 的根、种子和叶提取物中的葡萄糖硫苷（GLs）进行了特征描述和定量分析。通过黑芥子酶对 GL 降解生成的异硫氰酸酯（ITCs）进行 GC-MS 分析，对这些分析进行了补充。结果表明，根提取物中存在葡萄糖硫堇（1a）、葡萄糖辣根素（2a）和葡萄糖远志亭（3a），而种子中主要含有 3a。在叶提取物中检测到 3,4-二甲氧基苄基 GL（4a）、葡萄糖芸薹素（5a）和痕量的 1a。通过光谱技术将这些产物完全鉴定为其脱磺基对应物。
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