硫代葡萄糖苷谱和挥发性分离物的抗增殖潜力。

L.: Glucosinolate Profile and Antiproliferative Potential of Volatile Isolates.

机构信息

Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia.

Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia.

出版信息

Molecules. 2021 Aug 27;26(17):5183. doi: 10.3390/molecules26175183.

DOI:10.3390/molecules26175183

PMID:34500622

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8434519/

Abstract

Glucosinolates (GSLs) from L. were analyzed qualitatively and quantitatively by their desulfo-counterparts using UHPLC-DAD-MS/MS technique and by their volatile breakdown products-isothiocyanates (ITCs) using GC-MS analysis. Thirteen GSLs were identified with arylaliphatic as the major ones in the following order: 3-hydroxybenzyl GSL (glucolepigramin, ), benzyl GSL (glucotropaeolin, ), 3,4,5-trimethoxybenzyl GSL (), 3-methoxybenzyl GSL (glucolimnanthin, ), 4-hydroxy-3,5-dimethoxybenzyl GSL (3,5-dimethoxysinalbin, ), 4-hydroxybenzyl GSL (glucosinalbin, ), 3,4-dimethoxybenzyl GSL () and 2-phenylethyl GSL (gluconasturtiin, ). GSL breakdown products obtained by hydrodistillation (HD) and CHCl extraction after hydrolysis by myrosinase for 24 h (EXT) as well as benzyl ITC were tested for their cytotoxic activity using MTT assay. Generally, EXT showed noticeable antiproliferative activity against human bladder cancer cell line UM-UC-3 and human glioblastoma cell line LN229, and can be considered as moderately active, while IC of benzyl ITC was 12.3 μg/mL, which can be considered as highly active.

摘要

采用 UHPLC-DAD-MS/MS 技术分析其脱磺 counterparts和 GC-MS 分析其挥发性分解产物异硫氰酸酯 (ITC)，对 L.中的硫代葡萄糖苷 (GSL) 进行定性和定量分析。鉴定出 13 种以芳基脂肪族为主的 GSL，按以下顺序排列：3-羟基苄基 GSL (glucolepigramin, )、苄基 GSL (glucotropaeolin, )、3,4,5-三甲氧基苄基 GSL (), 3-甲氧基苄基 GSL (glucolimnanthin, )、4-羟基-3,5-二甲氧基苄基 GSL (3,5-二甲氧基西莱布辛, )、4-羟基苄基 GSL (glucosinalbin, )、3,4-二甲氧基苄基 GSL () 和 2-苯乙基 GSL (gluconasturtiin, )。通过水蒸馏 (HD) 和酶解 24 小时后用芥子酶提取的 CHCl 提取物 (EXT) 获得的 GSL 分解产物以及苄基 ITC ，通过 MTT 测定法测试其对人膀胱癌细胞系 UM-UC-3 和人神经胶质瘤细胞系 LN229 的细胞毒性活性。一般来说，EXT 对人膀胱癌细胞系 UM-UC-3 和人神经胶质瘤细胞系 LN229 表现出显著的抗增殖活性，可被认为是中度活性，而苄基 ITC 的 IC 为 12.3 μg/mL，可被认为是高度活性。