Centre for Watershed Ecology, Institute of Life Science, Nanchang University, People's Republic of China.
Curr Microbiol. 2012 Jan;64(1):34-42. doi: 10.1007/s00284-011-0031-1. Epub 2011 Oct 13.
To improve our understanding of the changes in bacterial and fungal diversity in natural pine and planted forests in subtropical region of China, we examined bacterial and fungal communities from a native and a nearby planted pine forest of the Mt. Lushan by constructing clone libraries of 16S and 18S rRNA genes. For bacterial communities, Proteobacteria and Acidobacteria were dominant bacterial taxa in both two types of forest soils. The Shannon-Wiener diversity index, rarefaction curve analysis, and LibShuff analysis suggest that these two forests contained similar diversity of bacterial communities. Low soil acidity (pH ≈ 4) of our study forests might be one of the most important selection factors determining growth of acidophilic Acidobacteria and Proteobacteria. However, the natural forest harbored greater level of fungal diversity than the planted forest according to the Shannon-Wiener diversity index and rarefaction curve analysis. Basidiomycota and Ascomycota were dominant fungal taxa in the soils of natural and planted forests, respectively. Our results suggest that fungal community was more sensitive than the bacterial community in characterizing the differences in plant cover impacts on the microbial flora in the natural and planted forests. The natural and planted forests may function differently due to the differences in soil fungal diversity and relative abundance.
为了增进我们对中国亚热带地区天然松林和人工种植林细菌和真菌多样性变化的理解，我们通过构建 16S 和 18S rRNA 基因克隆文库，研究了庐山本地和附近种植松林的细菌和真菌群落。对于细菌群落，在两种类型的森林土壤中，变形菌门和酸杆菌门都是主要的细菌分类群。香农-威纳多样性指数、稀疏曲线分析和 LibShuff 分析表明，这两个森林的细菌群落具有相似的多样性。我们研究的森林土壤酸度较低（pH≈4），这可能是决定嗜酸的酸杆菌和变形菌生长的最重要选择因素之一。然而，根据香农-威纳多样性指数和稀疏曲线分析，天然林的真菌多样性水平高于人工林。担子菌门和子囊菌门分别是天然林和人工林土壤中的主要真菌分类群。我们的研究结果表明，真菌群落比细菌群落更能敏感地描述植物覆盖度变化对天然林和人工林微生物区系的影响。由于土壤真菌多样性和相对丰度的差异，天然林和人工林的功能可能不同。
