Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA.
Appl Environ Microbiol. 2009 Dec;75(23):7537-41. doi: 10.1128/AEM.01541-09. Epub 2009 Oct 2.
mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the alpha and beta diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.
mothur 的目标是成为一个全面的软件包，允许用户使用单个软件来分析群落序列数据。它建立在以前的工具基础上，为分析测序数据提供了一个灵活强大的软件包。作为一个案例研究，我们使用 mothur 来修剪、筛选和对齐序列；计算距离；将序列分配到操作分类单元；并描述通过 16S rRNA 基因片段焦磷酸测序先前表征的八个海洋样本的 alpha 和 beta 多样性。使用笔记本电脑，在不到 2 小时的时间内完成了对超过 222,000 条序列的分析。
