Allison R T, Whittaker D K
University of Wales, College of Medicine, Dental School, Heath Park, Cardiff.
J Clin Pathol. 1990 Jul;43(7):600-3. doi: 10.1136/jcp.43.7.600.
Macroscopic evidence of bruising from human bite marks may be inconclusive and routine histochemical methods of showing extravasated erythrocytes can be unreliable. Leuco patent blue staining, for the presence of peroxidase, Amido black B, a tinctorial staining method for haemoglobin, Perls's reaction for ferric iron (haemosiderin), Masson-Fontana for melanin, Masson's trichrome, a connective tissue strain, and the benzidine reaction for haemoglobin peroxidase were carried out in three forensic cases and one experimental case. A modified benzidine method was the most reliable indicator of haemoglobin activity, especially where dispersion into extra-cellular tissues had occurred. The resilience of the erythrocyte peroxidase enzyme to temperature changes and fixation supports the concept of a "pseudo-peroxidase" in those cells. It is concluded that free haemoglobin from bite marks, or indeed other forms of blunt trauma, may best be shown by the benzidine reaction and that exemption certificates for use of this prohibited substance may be worth pursuing.
人类咬痕造成的瘀伤的宏观证据可能不明确，而显示红细胞外渗的常规组织化学方法可能不可靠。在三个法医案例和一个实验案例中，进行了用于检测过氧化物酶的无色专利蓝染色、用于检测血红蛋白的氨基黑B（一种染色方法）、用于检测三价铁（含铁血黄素）的佩尔斯反应、用于检测黑色素的马森-丰塔纳染色、用于检测结缔组织的马森三色染色以及用于检测血红蛋白过氧化物酶的联苯胺反应。改良的联苯胺方法是血红蛋白活性最可靠的指标，尤其是在血红蛋白已扩散到细胞外组织的情况下。红细胞过氧化物酶对温度变化和固定的耐受性支持了这些细胞中存在“假过氧化物酶”的概念。结论是，咬痕或其他形式钝器伤中的游离血红蛋白，最好通过联苯胺反应来显示，并且可能值得申请使用这种违禁物质的豁免证书。
