Slidders W, Hopwood D
Med Lab Sci. 1989 Jan;46(1):74-6.
The addition of 2% phenol has a marked adjuvant effect on 4% formaldehyde as a fixative. Sequential use of phenol formaldehyde buffered to pH 7.0 and pH 5.5 results in improved fixation in material rapidly advanced to paraffin in an enclosed tissue processor. Sections stained with alum haematoxylin and eosin show improved nuclear and cytoplasmic detail, reduced tissue shrinkage and an absence of formalin pigment. Excellent results are obtained with conventional special stains and immunoperoxidase methods.
添加2%的苯酚对4%的甲醛作为固定剂具有显著的辅助作用。依次使用pH值为7.0和pH值为5.5的苯酚甲醛缓冲液，能在封闭组织处理仪中快速将材料处理成石蜡块，从而改善固定效果。用明矾苏木精和伊红染色的切片显示细胞核和细胞质细节改善、组织收缩减少且无福尔马林色素。传统特殊染色和免疫过氧化物酶方法均取得了优异的结果。
