Exp Dermatol. 2011 Dec;20(12):1025-7. doi: 10.1111/j.1600-0625.2011.01381.x. Epub 2011 Oct 13.
To define the potential involvement of polymorphisms in the 3'untranslated region (3'UTR) of the prostaglandin synthetase-2 (PTGS-2) gene to non-melanoma skin cancer (NMSC) predisposition after transplantation, we screened for genetic variant, relevant parts of this region. It contains binding sites for trans-acting factors, an alternative polyadenylation site and putative target sequences for miRNAs. Variant +8473T>C did not appear to play a functional role in the regulation of gene expression in human keratinocyte-transfected cells. In addition to the well-known +8473T>C, we identified four polymorphisms: +8293G>C, +10259T>G, +10267G>A and +10335G>A. No allele frequency differences were observed between cases and controls neither for +8473T>C nor for any of the identified polymorphisms, suggesting that polymorphisms in the 3'UTR of the PTGS2 gene are rare and unlikely to represent risk factor for NMSC after transplantation.
为了确定前列腺素合成酶-2（PTGS-2）基因 3'非翻译区（3'UTR）中的多态性是否与移植后非黑色素瘤皮肤癌（NMSC）易感性有关，我们筛选了该区域的遗传变异相关部分。该区域包含反式作用因子结合位点、可变多聚腺苷酸化位点和miRNA 的潜在靶序列。变体+8473T>C 似乎没有在人角质形成细胞转染细胞中发挥调节基因表达的功能作用。除了众所周知的+8473T>C 之外，我们还鉴定了四个多态性：+8293G>C、+10259T>G、+10267G>A 和+10335G>A。在病例和对照组中，无论是+8473T>C 还是任何鉴定出的多态性，均未观察到等位基因频率差异，提示 PTGS2 基因 3'UTR 中的多态性很少，不太可能成为移植后 NMSC 的危险因素。
