Department of School of Public Health, Boston University, 715 Albany Street, Boston, MA 02118, USA.
Cell Biol Int. 2013 May;37(5):516-9. doi: 10.1002/cbin.10066. Epub 2013 Feb 27.
PTGS2 genetic 3' untranslated region (3'UTR) miRNA binding sites variants are significantly associated with cancer risk; however, the roles of genetic variants in PTGS2 gene 3'UTR and post-transcriptional regulation have not been elucidated. We report that rs689470 and rs5275 in the PTGS2 3'UTR have potential miRNA-binding sites by using bioinformatics analysis. However, only the rs689470 was significantly associated with PTGS2 mRNA expression in lymphoblastoid cell lines (P = 0.026), but not for rs5275 (P = 0.626). rs689470 might be putative variants mediating the post-transcriptional regulation of target PTGS2 gene. Better understanding of how 3'UTR variants regulate PTGS2 activity will pave the way to targeting the PTGS2 pathway in cancer therapy.
PTGS2 基因 3'UTR 内的遗传变异与癌症风险显著相关；然而，PTGS2 基因 3'UTR 内遗传变异的作用及其对转录后调控的影响尚未阐明。本研究通过生物信息学分析发现，PTGS2 基因 3'UTR 内 rs689470 和 rs5275 可能是 miRNA 的结合位点。然而，仅 rs689470 与淋巴母细胞系中 PTGS2 mRNA 表达显著相关（P=0.026），而 rs5275 则无显著相关性（P=0.626）。rs689470 可能是介导靶基因 PTGS2 转录后调控的假定变异。深入了解 3'UTR 变异如何调控 PTGS2 活性，将为癌症治疗中靶向 PTGS2 通路铺平道路。
