Hyodo A
Department of Orthopaedic Surgery, Yokohama City University School of Medicine, Kanagawa, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1990 Apr;64(4):282-91.
Histological changes in the ankle joints and serum anti-IgG rheumatoid factor-like substance detected by enzyme-linked immunosorbent assay (ELISA) were investigated in Sprague Dawley (SD), Wistar, and Lewis rats immunized with heat-killed Escherichia coli 0:14. Synovial lining cell hyperplasia was observed in 23 of 46 joints and pannus formation in 7 of 46 joints in SD rats immunized for 29 weeks. RFLS levels were elevated from the 15th, 7th, and 5th week of immunization in rats receiving doses of 0.1, 1, and 10 mg of E. coli respectively, and remained elevated dose-dependently. RFLS levels in SD and Wistar rats were significantly higher (p less than 0.01) than in Lewis rats during the period from the 7th to the 13th week of immunization. These results imply that immunization of rats with E. coli induces arthritic changes in the ankle joints and produces RFLS in the serum in a dose-dependent and different manner in each rat strain.
在用热灭活的大肠杆菌O:14免疫的斯普拉格-道利（SD）大鼠、Wistar大鼠和Lewis大鼠中，研究了踝关节的组织学变化以及通过酶联免疫吸附测定（ELISA）检测的血清抗IgG类风湿因子样物质。在免疫29周的SD大鼠中，46个关节中有23个观察到滑膜衬里细胞增生，46个关节中有7个观察到血管翳形成。分别接受0.1、1和10mg大肠杆菌剂量的大鼠在免疫第15、7和5周时RFLS水平升高，并呈剂量依赖性持续升高。在免疫第7至13周期间，SD大鼠和Wistar大鼠的RFLS水平显著高于Lewis大鼠（p小于0.01）。这些结果表明，用大肠杆菌免疫大鼠会诱导踝关节发生关节炎变化，并以剂量依赖性方式在各大鼠品系中使血清产生RFLS，且各品系有所不同。
