Respiratory Diseases Branch, Division of Bacterial Disease, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Diagn Microbiol Infect Dis. 2011 Dec;71(4):453-6. doi: 10.1016/j.diagmicrobio.2011.08.017. Epub 2011 Oct 11.
A quadriplex real-time polymerase chain reaction assay was developed for detecting pneumococci, penicillin susceptibility, and macrolide/lincosamide resistance. The assay was sensitive for all 4 targets (<10 copies) and correlated with antimicrobial susceptibilities in 172/180 isolates and 28/29 culture-positive clinical specimens. For 29 lytA-positive culture-negative specimens, the assay allowed interpretation of antimicrobial susceptibility.
研究开发了一种四重实时聚合酶链反应检测法，用于检测肺炎球菌、青霉素敏感性以及大环内酯类/林可酰胺类耐药性。该检测法对所有 4 个靶标（<10 个拷贝）均具有敏感性，与 172/180 株分离株和 28/29 份培养阳性临床标本的抗菌药物敏感性相吻合。对于 29 份 lytA 阳性但培养阴性的标本，该检测法可用于解释抗菌药物敏感性。
