四元实时聚合酶链反应（lytA、mef、erm、pbp2b[wt]）用于检测肺炎链球菌和评估抗生素敏感性。
Quadriplex real-time polymerase chain reaction (lytA, mef, erm, pbp2b(wt)) for pneumococcal detection and assessment of antibiotic susceptibility.
机构信息
Respiratory Diseases Branch, Division of Bacterial Disease, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
出版信息
Diagn Microbiol Infect Dis. 2011 Dec;71(4):453-6. doi: 10.1016/j.diagmicrobio.2011.08.017. Epub 2011 Oct 11.
A quadriplex real-time polymerase chain reaction assay was developed for detecting pneumococci, penicillin susceptibility, and macrolide/lincosamide resistance. The assay was sensitive for all 4 targets (<10 copies) and correlated with antimicrobial susceptibilities in 172/180 isolates and 28/29 culture-positive clinical specimens. For 29 lytA-positive culture-negative specimens, the assay allowed interpretation of antimicrobial susceptibility.
研究开发了一种四重实时聚合酶链反应检测法，用于检测肺炎球菌、青霉素敏感性以及大环内酯类/林可酰胺类耐药性。该检测法对所有 4 个靶标（<10 个拷贝）均具有敏感性，与 172/180 株分离株和 28/29 份培养阳性临床标本的抗菌药物敏感性相吻合。对于 29 份 lytA 阳性但培养阴性的标本，该检测法可用于解释抗菌药物敏感性。
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