Netsvyetayeva I, Sikora M, Golas M, Swoboda-Kopec E, de Walthoffen S Walter, Dembicka O, Fraczek M, Mlynarczyk A, Pacholczyk M, Chmura A, Mlynarczyk G
Department of Medical Microbiology, Warsaw Medical University, and Regional Hospital of Traumatology, St Anna, Warsaw, Poland.
Transplant Proc. 2011 Oct;43(8):3116-20. doi: 10.1016/j.transproceed.2011.07.008.
The increasing clinical significance of Acinetobacter baumannii species is due to its ability to survive in hospital environments, its species-specific multidrug resistance, and its ability to instantly develop various drug-resistance mechanisms through antibiotic pressure.
We identified 16 A baumannii strains isolated from patients presenting postoperative infections in 2010. A baumannii isolates were obtained from clinical specimens by standard microbiologic methods. As previously described, we performed polymerase chain reaction (PCR) analysis for carbapenemase-encoding genes (VIM, IMP, SPM, OXA23, OXA24, OXA51, OXA58) in Acinetobacter spp.
The double-disk synergy test phenotypic method did not detect any A baumannii strains producing metallo-beta-lactamaus cultured from swabs from all the patient groups. No products of PCR amplification with specific starters for VIM, IMP, and SPM (Sao Paulo metallo-β-lactamase) genes were found. All analyzed strains were colistin-sensitive. Among five strains from liver recipients, one was imipenem- and meropenem-resistant. Four among six strains isolated from cancer patients were resistant to imipenem and/or meropenem; 1/5 were imipenem-and meropenem-resistant; 1, meropenem-resistant and imipenem-sensitive; 1, meropenem- and imipenem-resistant; and 1 with intermediate resistance to both meropenem and imipenem among swabs cultured from patients with postoperative complication after bone fracture. Fifteen among 16 analyzed A baumannii strains had an OXA51 gene. Two among five A baumannii strains isolated in liver recipients had only an OXA51 gene; one, OXA51 and OXA24 genes; one, OXA51 and OXA23 genes.
鲍曼不动杆菌临床意义日益增加，这归因于其在医院环境中的生存能力、特定物种的多重耐药性以及通过抗生素压力迅速产生各种耐药机制的能力。
我们鉴定了2010年从术后感染患者中分离出的16株鲍曼不动杆菌菌株。鲍曼不动杆菌分离株通过标准微生物学方法从临床标本中获得。如前所述，我们对不动杆菌属中编码碳青霉烯酶的基因（VIM、IMP、SPM、OXA23、OXA24、OXA51、OXA58）进行了聚合酶链反应（PCR）分析。
双纸片协同试验表型方法未检测到从所有患者组拭子培养出的任何产金属β-内酰胺酶的鲍曼不动杆菌菌株。未发现用VIM、IMP和SPM（圣保罗金属β-内酰胺酶）基因特异性引物进行PCR扩增的产物。所有分析菌株对黏菌素敏感。在来自肝移植受者的5株菌株中，1株对亚胺培南和美罗培南耐药。从癌症患者中分离的6株菌株中有4株对亚胺培南和/或美罗培南耐药；骨折术后并发症患者拭子培养物中有五分之一对亚胺培南和美罗培南耐药；1株对美罗培南耐药且对亚胺培南敏感；1株对美罗培南和亚胺培南耐药；1株对美罗培南和亚胺培南均为中介耐药。16株分析的鲍曼不动杆菌菌株中有15株具有OXA51基因。在肝移植受者中分离的5株鲍曼不动杆菌菌株中有2株仅具有OXA51基因；1株具有OXA51和OXA24基因；1株具有OXA51和OXA23基因。
