Laboratory of Microengineering for Manufacturing, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Biosens Bioelectron. 2011 Dec 15;30(1):223-8. doi: 10.1016/j.bios.2011.09.016. Epub 2011 Sep 22.
We present a viscometric affinity biosensor for continuous monitoring of glucose in biological fluids such as blood and plasma. The sensing principle of this chemico-mechanical sensor is based upon the viscosity variation of a sensitive fluid with glucose concentration. Basically, this device includes both an actuating and a sensing piezoelectric diaphragms as well as a flow-resistive microchannel. In order to confine the sensitive fluid and allow glucose diffusion into the sensor, a free-standing alumina nanoporous membrane is also used as size-selective interface. Measurements carried out at nominal temperatures of 25 and 37 °C reveal that this sensor topology exhibits a high resolution in the current range of physiological blood glucose concentrations, i.e. 2-20 mM. In addition, complete reversibility was also demonstrated for at least 3 days. Finally, measurements performed in human blood serum confirm that this sensor fulfils all basic requirements for a use in continuous glucose monitoring of biological fluids.
我们提出了一种用于在血液和血浆等生物流体中连续监测葡萄糖的黏度亲和生物传感器。该化学机械传感器的传感原理基于敏感流体的粘度随葡萄糖浓度的变化。基本上，该装置包括一个致动和一个传感压电膜片以及一个流动阻力微通道。为了限制敏感流体并允许葡萄糖扩散到传感器中，还使用了独立式氧化铝纳米多孔膜作为尺寸选择性界面。在名义温度为 25 和 37°C 下进行的测量表明，这种传感器拓扑结构在生理血糖浓度范围内（即 2-20mM）具有很高的分辨率。此外，至少 3 天的时间内还证明了完全的可逆性。最后，在人血清中的测量结果证实，该传感器完全满足在生物流体中连续监测葡萄糖的所有基本要求。
