Department of Cellular and Environmental Biology, University of Perugia, Italy.
Bioresour Technol. 2011 Dec;102(23):10965-72. doi: 10.1016/j.biortech.2011.09.062. Epub 2011 Sep 20.
In this study, physico-chemical modifications and community dynamics and functional role of the resident microbiota during composting of humid husk from a two-phase extraction system (TPOMW) were investigated. High mineralization and humification of carbon, low loss of nitrogen and complete degradation of polyphenols led to the waste biotransformation into a high-quality compost. Viable cell counts and denaturing gradient gel electrophoresis (DGGE) profiling of the 16S rRNA genes showed that the thermophilic phase was characterized by the strongest variations of cell number, the highest biodiversity and the most variable community profiles. The isolation of tannin-degrading bacteria (e.g. Lysinibacillus fusiformis, Kocuria palustris, Tetrathiobacter kashmirensis and Rhodococcus rhodochrous) suggested a role of this enzymatic activity during the process. Taken together, the results indicated that the composting process, particularly the thermophilic phase, was characterized by a rapid succession of specialized bacterial populations with key roles in the organic matter biotransformation.
本研究考察了在两相萃取系统（TPOMW）的湿壳废物堆肥过程中物理化学修饰以及定居微生物群落动态和功能作用。碳的高度矿化和腐殖化、氮的低损失和多酚的完全降解导致废物生物转化为高质量堆肥。可培养细胞计数和 16S rRNA 基因的变性梯度凝胶电泳（DGGE）分析表明，高温期的细胞数量变化最大，生物多样性最高，群落结构变化最大。单宁降解菌（如溶纤维芽孢杆菌、沼泽红假单胞菌、喀什米尔硫杆菌和红球菌）的分离表明，该酶活性在该过程中发挥了作用。总之，结果表明，堆肥过程，特别是高温期，具有特定细菌种群的快速演替，这些种群在有机质生物转化中起着关键作用。
