Department of Biology, Federal University of Lavras (UFLA), Lavras, MG, 37200-000, Brazil.
Department of Hydraulic and Environmental Engineering, Federal University of Ceará, Fortaleza, CE, 60455-900, Brazil.
Biodegradation. 2021 Oct;32(5):551-562. doi: 10.1007/s10532-021-09951-0. Epub 2021 May 27.
In this work, strains of Bacillus subtilis were inoculated in consortium with Rhodotorula mucilaginosa into spent soy oil as aiming to biological treatment and low-cost reuse. The microorganisms were previously isolated and selected for the lipolytic capacity of the alperujo residue generated during the processing of olive oil. For fermentation, bioassays containing Rhodotorula mucilaginosa isolated from alperujo and Candida rugosa CCMA 00371, both co-inoculated with Bacillus subtilis CCMA 0085 in medium containing (% w/v) 0.075 glucose and 0.375 (NH) PO in 75 mL of water and 75 mL of spent soy oil. Despite the low biomass productivity, it has favorable characteristics to be used in animal feed supplementation. Spent soy oil was used as a carbon source proven by Bartha respirometer. The strains of R. mucilaginosa UFLA RAS 144 and B. subtilis CCMA 0085 are promising inoculants for oil degradation and can be applied in a waste treatment system.
在这项工作中，枯草芽孢杆菌菌株与粘红酵母接种于用过的大豆油中，旨在进行生物处理和低成本再利用。这些微生物是先前从橄榄油加工过程中产生的油渣中分离和选择出来的，具有脂肪酶活性。用于发酵的生物测定含有从油渣中分离出的粘红酵母和 Candida rugosa CCMA 00371，两者均与枯草芽孢杆菌 CCMA 0085 共同接种于含有（% w/v）0.075 葡萄糖和 0.375（NH）PO 的培养基中，在 75 毫升水中和 75 毫升用过的大豆油中。尽管生物量生产力较低，但它具有作为动物饲料添加剂的有利特性。用过的大豆油被证明是一种碳源，可通过巴氏呼吸计进行验证。粘红酵母 UFLA RAS 144 和枯草芽孢杆菌 CCMA 0085 菌株是具有降解油脂潜力的接种物，可应用于废物处理系统。
