Soyupek Sedat, Oksay Taylan, Sütçü Recep, Armagan Abdullah, Gökalp Osman, Perk Hakki, Delibas Namik
Department of Urology, School of Medicine, Suleyman Demirel University, Isparta, Turkey.
Toxicol Ind Health. 2012 Aug;28(7):624-8. doi: 10.1177/0748233711420467. Epub 2011 Oct 13.
The aim of this study was to assess the cadmium (Cd) toxicity on renal nitric oxide synthase (NOS) isoenzymes. The study was carried out on 18 inbred male (Cd group: 10 and control group: 8) Wistar rats. Cd group received drinking water containing 15 mg/L Cd for 30 days; and at the end of the 30 days, plasma Cd was analysed. One kidney was snap frozen to assess the endothelial NOS (eNOS), inducible NOS (iNOS) and neuronal NOS (nNOS) expressions by Western blot analyses, and the other kidney was preserved for histopathological examination. Plasma Cd levels were significantly elevated in the Cd group. The Western blot analyses found higher levels of eNOS, iNOS and nNOS in the Cd group but only eNOS and nNOS levels were statistically significant. There was no difference in pathological assessment of the renal tissues. Cd toxicity increases NOS isoenzyme levels and may affect renal physiology.
本研究的目的是评估镉（Cd）对肾脏一氧化氮合酶（NOS）同工酶的毒性作用。该研究以18只近交系雄性Wistar大鼠进行（镉组：10只，对照组：8只）。镉组大鼠饮用含15 mg/L镉的水30天；在30天结束时，分析血浆镉含量。将一侧肾脏速冻，通过蛋白质免疫印迹分析评估内皮型一氧化氮合酶（eNOS）、诱导型一氧化氮合酶（iNOS）和神经元型一氧化氮合酶（nNOS）的表达，另一侧肾脏留作组织病理学检查。镉组血浆镉水平显著升高。蛋白质免疫印迹分析发现镉组中eNOS、iNOS和nNOS水平较高，但只有eNOS和nNOS水平具有统计学意义。肾脏组织的病理学评估没有差异。镉毒性增加了NOS同工酶水平，可能会影响肾脏生理功能。
