Department of Animal Physiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Department of Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Environ Sci Pollut Res Int. 2017 Nov;24(32):25475-25481. doi: 10.1007/s11356-017-0348-9. Epub 2017 Oct 3.
Biochemical and histopathological effects of subacute intoxication of rats with cadmium (Cd) were studied in rats. Twenty adult healthy male albino rats were randomly divided into two duplicate groups (five rats in each cage); (1) control group where rats were provided with standard diet and water ad-libitum, (2) Cd group where rats were subjected to freshly prepared Cd chloride solution (CdCl2) 200 mg/l in drinking water daily for 8 weeks, the whole duration of experiment. Blood samples were obtained after 4 weeks, via retro-orbital bleeding for separation of serum. Five rats were killed, each sacrifice by decapitation for collection of kidneys and heart. Disturbed renal and cardiac functions were achieved after 4 weeks as indicated by the increase of most biochemical parameters measured in the serum, renal, and cardiac tissues. Histopathological examination of kidneys and hearts showed pathological alterations in Cd-intoxicated rats after 4 and 8 weeks with hematoxylin and eosin (H&E) and Masson trichrome stains. It was concluded that subacute exposure of rats to Cd (200 mg/l) in drinking water daily induced glomerular shrinkage, focal renal, and cardiac fibrosis at 4 and 8 weeks.
研究了亚急性镉（Cd）中毒对大鼠的生化和组织病理学影响。将 20 只成年健康雄性白化大鼠随机分为两组（每组 5 只）：（1）对照组，大鼠提供标准饮食和自由饮水；（2）Cd 组，大鼠每天饮用新鲜配制的 CdCl2 200mg/L 水溶液，持续 8 周。第 4 周末通过眶后采血分离血清。处死 5 只大鼠，每只通过断头法采集肾脏和心脏。4 周后，血清、肾脏和心脏组织中大多数生化参数的升高表明肾脏和心脏功能受到干扰。苏木精和伊红（H&E）和 Masson 三色染色显示，4 周和 8 周后，Cd 染毒大鼠的肾脏和心脏出现组织病理学改变。结论：大鼠每日经饮水摄入 200mg/L 的 Cd（200mg/L），在 4 周和 8 周时会导致肾小球缩小、局灶性肾和心脏纤维化。
