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用于测量折射率的图像对比度浸没法应用于蜘蛛丝。

Image contrast immersion method for measuring refractive index applied to spider silks.

作者信息

Little Douglas J, Kane D M

机构信息

MQ Photonics Research Centre, Department of Physics and Astronomy, Macquarie University, Sydney, NSW 2109, Australia.

出版信息

Opt Express. 2011 Sep 26;19(20):19182-9. doi: 10.1364/OE.19.019182.

DOI:10.1364/OE.19.019182
PMID:21996860
Abstract

A technique for measuring the refractive index of micron sized fibers using a series of immersion index matching oils, and image contrast measurements is proposed and demonstrated. It has been applied to radial silks of the orb web weaving spider Plebs eburnus. These have widths of ~1-2 microns. Values about 1.5500 are obtained, with birefringence values between 0.0000 and 0.0133 for individual silks. An uncertainty in the range ± 5 × 10(-4) to ± 2 × 10(-3) is achieved for these challenging samples. This accuracy is about a twenty times improvement on previously reported measurements for spider silks using other techniques. The technique is used to obtain measurements of the refractive index of spider silks as a function of wavelength, for the first time. An Abbe number for the radial silks of Plebs eburnus of ~32 is found.

摘要

提出并演示了一种使用一系列浸没折射率匹配油和图像对比度测量来测量微米级纤维折射率的技术。该技术已应用于织圆网的蜘蛛Plebs eburnus的径向丝。这些丝的宽度约为1-2微米。获得的值约为1.5500,单根丝的双折射值在0.0000至0.0133之间。对于这些具有挑战性的样品,实现了±5×10(-4)至±2×10(-3)范围内的不确定度。这种精度比以前使用其他技术报道的蜘蛛丝测量精度提高了约二十倍。该技术首次用于获得蜘蛛丝折射率随波长变化的测量结果。发现Plebs eburnus径向丝的阿贝数约为32。

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