Gyger Markus, Rose Daniel, Stange Roland, Kiessling Tobias, Zink Mareike, Fabry Ben, Käs Josef A
Institut für Experimentelle Physik I, Abteilung für Physik der Weichen Materie, Universität Leipzig, Linnéstr. 5, 04103 Leipzig, Germany.
Opt Express. 2011 Sep 26;19(20):19212-22. doi: 10.1364/OE.19.019212.
The Microfluidic Optical Stretcher (MOS) has previously been shown to be a versatile tool to measure mechanical properties of single suspended cells. In this study we combine optical stretching and fluorescent calcium imaging. A cell line transfected with a heat sensitive cation channel was used as a model system to show the versatility of the setup. The cells were loaded with the Ca(2+) dye Fluo-4 and imaged with confocal laser scanning microscopy while being stretched. During optical stretching heat is transferred to the cell causing a pronounced Ca(2+) influx through the cation channel. The technique opens new perspectives for investigating the role of Ca(2+) in regulating cell mechanical behavior.
微流控光学拉伸仪（MOS）此前已被证明是一种用于测量单个悬浮细胞力学特性的多功能工具。在本研究中，我们将光学拉伸与荧光钙成像相结合。使用转染了热敏阳离子通道的细胞系作为模型系统，以展示该装置的多功能性。细胞加载Ca(2+)染料Fluo-4，并在拉伸过程中用共聚焦激光扫描显微镜成像。在光学拉伸过程中，热量传递到细胞，导致Ca(2+)通过阳离子通道大量内流。该技术为研究Ca(2+)在调节细胞力学行为中的作用开辟了新的视角。
