Yang Tie, Bragheri Francesca, Minzioni Paolo
Department of Electrical, Computer, and Biomedical Engineering, Università di Pavia, Via Ferrata 5A, Pavia 27100, Italy.
Institute of Photonics and Nanotechnology, CNR & Department of Physics, Politecnico di Milano, Piazza Leonardo da Vinci 32, Milano 20133, Italy.
Micromachines (Basel). 2016 May 13;7(5):90. doi: 10.3390/mi7050090.
This paper presents a comprehensive review of the development of the optical stretcher, a powerful optofluidic device for single cell mechanical study by using optical force induced cell stretching. The different techniques and the different materials for the fabrication of the optical stretcher are first summarized. A short description of the optical-stretching mechanism is then given, highlighting the optical force calculation and the cell optical deformability characterization. Subsequently, the implementations of the optical stretcher in various cell-mechanics studies are shown on different types of cells. Afterwards, two new advancements on optical stretcher applications are also introduced: the active cell sorting based on cell mechanical characterization and the temperature effect on cell stretching measurement from laser-induced heating. Two examples of new functionalities developed with the optical stretcher are also included. Finally, the current major limitation and the future development possibilities are discussed.
本文全面综述了光镊的发展，光镊是一种强大的光流体装置，用于通过光力诱导细胞拉伸进行单细胞力学研究。首先总结了制造光镊的不同技术和不同材料。然后简要描述了光镊拉伸机制，重点介绍了光力计算和细胞光学变形性表征。随后，展示了光镊在各种细胞力学研究中对不同类型细胞的应用。之后，还介绍了光镊应用的两项新进展：基于细胞力学表征的主动细胞分选以及激光诱导加热对细胞拉伸测量的温度效应。文中还包括了利用光镊开发的两项新功能示例。最后，讨论了当前的主要局限性和未来的发展可能性。
