加拿大安大略省 2008 年玉米作物中游离脱氧雪腐镰刀菌烯醇和结合脱氧雪腐镰刀菌烯醇的调查。

A survey of free and conjugated deoxynivalenol in the 2008 corn crop in Ontario, Canada.

机构信息

Department of Animal and Poultry Science, University of Guelph, Guelph, ON N1G 2 W1, Canada.

出版信息

J Sci Food Agric. 2012 Jan 15;92(1):37-41. doi: 10.1002/jsfa.4674. Epub 2011 Oct 14.

DOI:10.1002/jsfa.4674

PMID:21997524

Abstract

BACKGROUND

Deoxynivalenol (DON, vomitoxin), one of the most important mycotoxins produced by many Fusarium species, is found as a common contaminant of crops worldwide. Recent studies have described the presence of conjugated forms of DON (glycosides and fatty acid). The aim of the current study was therefore to investigate the natural occurrence of free and conjugated DON in Canadian corn.

RESULTS

Free and conjugated DON was determined by gas chromatography-mass spectrometry (GC-MS) and enzyme-linked immunosorbent assay (ELISA) in 86 corn samples collected from the 2008 crop in Ontario, Canada. Free DON concentrations determined by ELISA were similar to values determined in most samples using GC-MS. Conjugated DON was detected in 72 samples. Levels of free DON ranged from 0.17 to 14.00 µg g(-1) using GC-MS. The highest levels of free DON were found in corn samples from the southern and southwestern regions of Ontario, while samples from eastern regions were less contaminated. Conjugated DON was found mainly in corn from the east-central region, with five of six samples showing high levels of conjugated DON (up to 43% increase in DON following acid hydrolysis). Low levels of conjugated DON (≤ 10% increase in DON following acid hydrolysis) were detected in the majority of corn samples from the southwestern region (nine of 19 samples) and from the central region (16 of 36 samples).

CONCLUSION

The current survey emphasizes the frequency of conjugated DON in Ontario grown corn and the potential challenges in understanding the hazard posed by DON-contaminated foodstuffs and feedstuffs.

摘要

背景

脱氧雪腐镰刀菌烯醇（DON，呕吐毒素）是由许多镰刀菌属产生的最重要的霉菌毒素之一，是全世界作物中常见的污染物。最近的研究描述了 DON 的共轭形式（糖苷和脂肪酸）的存在。因此，本研究旨在调查加拿大玉米中游离和共轭 DON 的自然发生情况。

结果

通过气相色谱-质谱联用（GC-MS）和酶联免疫吸附测定（ELISA）在加拿大安大略省 2008 年作物中采集的 86 个玉米样本中测定了游离和共轭 DON。ELISA 测定的游离 DON 浓度与大多数使用 GC-MS 测定的样品值相似。在 72 个样品中检测到共轭 DON。使用 GC-MS 测定，游离 DON 的浓度范围为 0.17 至 14.00 µg g(-1)。游离 DON 的最高水平出现在安大略省南部和西南部的玉米样本中，而来自东部地区的样本污染较少。共轭 DON 主要存在于来自中东部地区的玉米中，其中六个样品中有五个表现出高共轭 DON 水平（酸水解后 DON 增加了 43%）。在西南部（19 个样本中的 9 个）和中部地区（36 个样本中的 16 个）的大多数玉米样本中检测到低水平的共轭 DON（酸水解后 DON 增加了 ≤ 10%）。