Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Vic., Australia.
Respiration. 2012;83(3):253-66. doi: 10.1159/000330586. Epub 2011 Oct 11.
Assessment of deterioration of lung function in animal models of respiratory disease traditionally relies upon quantitating biochemical markers. Plethysmography is a technique for measuring lung function that includes invasive and non-invasive methodologies.
This study used whole-body barometric plethysmography to characterize change(s) in respiratory physiology of C57BL/6 mice following bleomycin administration.
Cohorts of animals were culled at 3, 7, 14 and 28 days to semi-quantitatively score the lung for fibrosis, and quantitate levels of hydroxyproline in the lung. We have described in detail the response of C57BL/6 mice to bleomycin.
Bleomycin-treated mice had reduced minute volume (p < 0.05) and an increased total breathing cycle time (p < 0.0001), which consisted of a shortened inspiration time (p < 0.01) and an extended expiration time (p < 0.0001).
We have demonstrated that plethysmography can be a primary indicator of the development of respiratory disease in the mouse and would thus be suitable in assessing potential therapies since any truly effective treatment should elicit restoration of respiratory parameters in addition to improving traditional biochemical and histological indices of lung function.
传统上，评估呼吸道疾病动物模型中肺功能的恶化依赖于定量生化标志物。体积描记法是一种测量肺功能的技术，包括有创和无创方法。
本研究使用全身气压体积描记法来描述博莱霉素给药后 C57BL/6 小鼠呼吸生理的变化。
动物队列在第 3、7、14 和 28 天被处死，以半定量方式对肺纤维化进行评分，并定量测定肺羟脯氨酸水平。我们详细描述了 C57BL/6 小鼠对博莱霉素的反应。
博莱霉素处理的小鼠分钟通气量减少（p<0.05），总呼吸周期时间延长（p<0.0001），包括吸气时间缩短（p<0.01）和呼气时间延长（p<0.0001）。
我们已经证明，体积描记法可以作为小鼠呼吸道疾病发展的主要指标，因此适合评估潜在的治疗方法，因为任何真正有效的治疗方法都应该在改善肺功能的传统生化和组织学指标的基础上，恢复呼吸参数。
