Martin-Luther-Universität Halle-Wittenberg, Bereich Organische Chemie, Halle (Saale), Germany.
Arch Pharm (Weinheim). 2012 Mar;345(3):223-30. doi: 10.1002/ardp.201100046. Epub 2011 Oct 14.
The extracts of the roots of licorice have been used in traditional and folk medicine to treat a broad variety of maladies. The main ingredient of these extracts is glycyrrhicinic acid. Its aglycon, glycyrrhetinic acid, has many biological activities, among them a pronounced cytotoxicity against tumor cells. In this study we varied glycyrrhetinic acid at position C-30 to get "simple" derivatives, for example esters, amides and a nitrile. The influence of these changes on the cytotoxic activity is noteworthy and was determined by a colorimetric sulphorhodamine B test using 7 human tumor cell lines and mouse embryonic fibroblasts (NIH3T3) for comparison. A Trypan blue test as well as an acridine orange/ethidium bromide test was used to discover the ability of the compounds to induce apoptosis.
甘草根提取物在传统和民间医学中被用于治疗多种疾病。这些提取物的主要成分是甘草酸。其苷元甘草次酸具有许多生物活性，其中对肿瘤细胞具有明显的细胞毒性。在这项研究中，我们在 C-30 位改变了甘草次酸，得到了“简单”的衍生物，例如酯、酰胺和腈。这些变化对细胞毒性的影响值得注意，并通过使用 7 个人类肿瘤细胞系和小鼠胚胎成纤维细胞（NIH3T3）进行比较的比色磺基罗丹明 B 试验来确定。使用台盼蓝试验和吖啶橙/溴化乙锭试验来发现化合物诱导细胞凋亡的能力。
