HPLC Group, Analytical Chemistry Division, Discovery Laboratory Indian Institute of Chemical Technology (IICT), Tarnaka, Hyderabad, Andhra Pradesh, India.
J Sep Sci. 2011 Nov;34(22):3265-71. doi: 10.1002/jssc.201100560. Epub 2011 Oct 14.
A novel water-compatible molecularly imprinted SPE combined with zwitterionic hydrophilic interaction liquid chromatography method for selective extraction and determination of 3-methylflavone-8-carboxylic acid (MFA), the main active metabolite of flavoxate in human urine, was developed and validated. The effects of progenic solvents, pH, cross linker and amount of monomer were studied to optimize the efficiency and selectivity. The molecularly imprinted polymer showed good specific adsorption capacity with an optimum of 200 μmol/g at pH 7.5 and selective extraction of MFA from human urine. The recovery of MFA from human urine was >98%. The lower limit of quantification was 1.20 μg/mL. The proposed method overcomes the matrix effects of endogenous substances generally encountered during direct analysis of urine sample.
建立了一种新型的水相兼容分子印迹固相萃取-两性离子亲水作用色谱联用方法，用于选择性提取和测定尿液中主要的黄酮哌酯代谢产物 3-甲基黄烷酮-8-羧酸（MFA）。考察了衍生溶剂、pH 值、交联剂和单体用量对印迹效率和选择性的影响。印迹聚合物在 pH 值为 7.5 时对 MFA 具有最佳的吸附容量（200μmol/g），对 MFA 具有良好的特异性。MFA 从人尿液中的回收率大于 98%。定量下限为 1.20μg/mL。该方法克服了直接分析尿液样品时通常遇到的内源性物质的基质效应。
