Hackel C, Houard S, Portaels F, van Elsen A, Herzog A, Bollen A
Service for Applied Genetics, Université Libre de Bruxelles, Nivelles, Belgium.
Mol Cell Probes. 1990 Jun;4(3):205-10. doi: 10.1016/0890-8508(90)90054-4.
Oligonucleotide primers have been used to amplify DNA regions of the M. leprae genome by the polymerase chain reaction. A first set of primers, PLp1 and PLp2, identifies a specific 386 bp DNA fragment located in the gene coding for the 65 kDa antigen of M. leprae. A second pair of primers, targetted to the same gene, leads to the amplification of a 154 bp DNA piece conserved in mycobacteria. Primers PLp1 and PLp2 discriminate the pathogenic species from other mycobacteria, detect down to 40 bacilli, and constitute potentially useful tools for the identification of M. leprae in clinical specimens.
寡核苷酸引物已被用于通过聚合酶链反应扩增麻风分枝杆菌基因组的DNA区域。第一组引物PLp1和PLp2可识别位于编码麻风分枝杆菌65 kDa抗原的基因中的一个特定386 bp DNA片段。针对同一基因的第二对引物可扩增出分枝杆菌中保守的154 bp DNA片段。引物PLp1和PLp2可将致病菌种与其他分枝杆菌区分开来，能检测低至40条杆菌，是鉴定临床标本中麻风分枝杆菌的潜在有用工具。
