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运用聚合酶链反应和特异性DNA探针检测未治疗的分枝杆菌。

Detection of untreated mycobacteria by using polymerase chain reaction and specific DNA probes.

作者信息

Fries J W, Patel R J, Piessens W F, Wirth D F

机构信息

Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts 02115.

出版信息

J Clin Microbiol. 1991 Aug;29(8):1744-7. doi: 10.1128/jcm.29.8.1744-1747.1991.

DOI:10.1128/jcm.29.8.1744-1747.1991
PMID:1761699
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC270197/
Abstract

A method for specific identification of mycobacteria by using the polymerase chain reaction on organisms taken from liquid cultures, frozen suspensions, or colonies grown on Lowenstein-Jensen slants is presented. This direct detection of mycobacterial organisms has important implications for strain typing and diagnosis.

摘要

本文介绍了一种通过聚合酶链反应对取自液体培养物、冷冻悬液或在罗-琴斜面培养基上生长的菌落中的微生物进行分枝杆菌特异性鉴定的方法。这种对分枝杆菌微生物的直接检测对菌株分型和诊断具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aa0/270197/efa44a1c300e/jcm00044-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aa0/270197/324b8ad72a53/jcm00044-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aa0/270197/6735cf865a25/jcm00044-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aa0/270197/efa44a1c300e/jcm00044-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aa0/270197/324b8ad72a53/jcm00044-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aa0/270197/6735cf865a25/jcm00044-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aa0/270197/efa44a1c300e/jcm00044-0212-a.jpg

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本文引用的文献

1
Repetitive DNA sequence of Mycobacterium tuberculosis: analysis of differential hybridization pattern with other mycobacteria.结核分枝杆菌的重复DNA序列：与其他分枝杆菌的差异杂交模式分析
Int J Lepr Other Mycobact Dis. 1988 Dec;56(4):592-8.
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Use of a cutoff range in identifying mycobacteria by the Gen-Probe Rapid Diagnostic System.在使用Gen-Probe快速诊断系统鉴定分枝杆菌时使用截断范围。
J Clin Microbiol. 1989 Feb;27(2):241-4. doi: 10.1128/jcm.27.2.241-244.1989.
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The use of DNA probes identifying restriction-fragment-length polymorphisms to examine the Mycobacterium avium complex.
用于培养结核分枝杆菌的具有成本效益的总核酸提取方案的评估；通过对与耐药性相关基因的PCR扩增进行比较。
BMC Res Notes. 2010 Feb 26;3:48. doi: 10.1186/1756-0500-3-48.
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Rapid diagnosis of extrapulmonary tuberculosis by PCR: impact of sample preparation and DNA extraction.通过聚合酶链反应快速诊断肺外结核病：样本制备和DNA提取的影响
J Clin Microbiol. 2003 Jun;41(6):2323-9. doi: 10.1128/JCM.41.6.2323-2329.2003.
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Detection and identification of Mycobacterium avium in the blood of AIDS patients by the polymerase chain reaction.通过聚合酶链反应检测和鉴定艾滋病患者血液中的鸟分枝杆菌
Eur J Clin Microbiol Infect Dis. 1996 Jul;15(7):551-5. doi: 10.1007/BF01709362.
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Preparation of mycobacterial DNA from blood culture fluids by simple alkali wash and heat lysis method for PCR detection.通过简单碱洗和热裂解方法从血培养液中制备用于PCR检测的分枝杆菌DNA。
J Clin Microbiol. 1996 Aug;34(8):1985-91. doi: 10.1128/jcm.34.8.1985-1991.1996.
7
Nucleotide sequence analysis and serologic characterization of a 27-kilodalton Mycobacterium intracellulare lipoprotein.27千道尔顿细胞内分枝杆菌脂蛋白的核苷酸序列分析及血清学特性鉴定
Infect Immun. 1993 Mar;61(3):1074-81. doi: 10.1128/iai.61.3.1074-1081.1993.
8
Detection of Mycobacterium tuberculosis in clinical samples by two-step polymerase chain reaction and nonisotopic hybridization methods.采用两步聚合酶链反应和非同位素杂交法检测临床样本中的结核分枝杆菌。
J Clin Microbiol. 1993 Jan;31(1):61-5. doi: 10.1128/jcm.31.1.61-65.1993.
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Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples.用于检测临床样本中结核分枝杆菌的巢式聚合酶链反应
J Clin Microbiol. 1993 Aug;31(8):2228-32. doi: 10.1128/jcm.31.8.2228-2232.1993.
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Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory.在常规分枝杆菌学实验室中大规模使用聚合酶链反应检测结核分枝杆菌。
J Clin Microbiol. 1993 Aug;31(8):2049-56. doi: 10.1128/jcm.31.8.2049-2056.1993.
使用鉴定限制性片段长度多态性的DNA探针来检测鸟分枝杆菌复合体。
Mol Microbiol. 1987 Nov;1(3):283-91. doi: 10.1111/j.1365-2958.1987.tb01934.x.
4
Identification and partial characterization of Mycobacterium avium and Mycobacterium intracellulare by using DNA probes.利用DNA探针鉴定鸟分枝杆菌和胞内分枝杆菌并进行部分特性分析。
J Clin Microbiol. 1989 May;27(5):994-7. doi: 10.1128/jcm.27.5.994-997.1989.
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Polymerase chain reaction for the detection of Mycobacterium leprae.用于检测麻风分枝杆菌的聚合酶链反应
J Gen Microbiol. 1989 Sep;135(9):2357-64. doi: 10.1099/00221287-135-9-2357.
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Mycobacterial disease in patients infected with the human immunodeficiency virus.感染人类免疫缺陷病毒患者的分枝杆菌病
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Polymorphic repetitive DNA sequences in Mycobacterium tuberculosis detected with a gene probe from a Mycobacterium fortuitum plasmid.用偶然分枝杆菌质粒的基因探针检测结核分枝杆菌中的多态性重复DNA序列。
J Gen Microbiol. 1989 Sep;135(9):2347-55. doi: 10.1099/00221287-135-9-2347.
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Rapid diagnosis of tuberculosis by amplification of mycobacterial DNA in clinical samples.通过扩增临床样本中的分枝杆菌DNA快速诊断结核病
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DNA probes demonstrate a single highly conserved strain of Mycobacterium avium infecting AIDS patients.DNA探针显示，有一种单一的高度保守的鸟分枝杆菌菌株正在感染艾滋病患者。
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