运用聚合酶链反应和特异性DNA探针检测未治疗的分枝杆菌。
Detection of untreated mycobacteria by using polymerase chain reaction and specific DNA probes.
作者信息
Fries J W, Patel R J, Piessens W F, Wirth D F
机构信息
Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts 02115.
出版信息
J Clin Microbiol. 1991 Aug;29(8):1744-7. doi: 10.1128/jcm.29.8.1744-1747.1991.
Abstract
A method for specific identification of mycobacteria by using the polymerase chain reaction on organisms taken from liquid cultures, frozen suspensions, or colonies grown on Lowenstein-Jensen slants is presented. This direct detection of mycobacterial organisms has important implications for strain typing and diagnosis.
摘要
本文介绍了一种通过聚合酶链反应对取自液体培养物、冷冻悬液或在罗-琴斜面培养基上生长的菌落中的微生物进行分枝杆菌特异性鉴定的方法。这种对分枝杆菌微生物的直接检测对菌株分型和诊断具有重要意义。
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