Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84132-2408, USA.
J Immunol. 2011 Nov 15;187(10):5203-10. doi: 10.4049/jimmunol.1102119. Epub 2011 Oct 12.
The details of the bifurcation of the lymphoid and myeloid lineages following commitment by multipotent progenitor cells (MPP) remain a topic of controversy. We report that the surface glycoprotein CD62L can be characterized as a novel marker of this and other stages of early hematopoietic differentiation. Cell isolation and transplant studies demonstrated CD62L(neg/low) long-term hematopoietic stem cells and CD62L(high) MPP within the traditionally defined c-kit(pos)Lin(neg/low)Sca-1(pos) stem/progenitor cell population. Within the MPP population, previously defined as c-kit(pos)Lin(neg/low)Sca-1(pos)-Thy-1.1(neg)Flt3(pos), Sca-1 and CD62L resolved four populations and segregated Sca-1(high)CD62L(neg/low) MPP from Sca-1(high)CD62L(high) leukocyte-biased progenitors. Using a novel transplantation method that allows tracking of erythroid and platelet engraftment as an alternative to the classical method of in vitro colony formation, we characterized Sca-1(high)CD62L(neg/low) cells as MPP, based on transient engraftment of these lineages. These data establish CD62L as a useful tool in the study of early hematopoiesis and emphasize the power of trilineage-engraftment studies in establishing the lineage potential of MPP subsets.
多能祖细胞（MPP）定向分化为淋巴系和髓系细胞的详细过程仍然存在争议。我们报告表面糖蛋白 CD62L 可以作为该过程和其他早期造血分化阶段的新型标志物。细胞分离和移植研究表明，CD62L(neg/low) 长期造血干细胞和 CD62L(high) MPP 位于传统定义的 c-kit(pos)Lin(neg/low)Sca-1(pos)干细胞/祖细胞群内。在 MPP 群体中，之前被定义为 c-kit(pos)Lin(neg/low)Sca-1(pos)-Thy-1.1(neg)Flt3(pos)，Sca-1 和 CD62L 可将群体分为四个亚群，并将 Sca-1(high)CD62L(neg/low) MPP 与 Sca-1(high)CD62L(high)偏向白细胞的祖细胞分开。我们使用一种新的移植方法，允许追踪红系和血小板植入，作为替代经典的体外集落形成方法，将 Sca-1(high)CD62L(neg/low)细胞鉴定为 MPP，基于这些谱系的短暂植入。这些数据确立了 CD62L 作为研究早期造血的有用工具，并强调了三系植入研究在确定 MPP 亚群谱系潜力方面的力量。
