Perry S Scott, Wang Hongfang, Pierce L Jeanne, Yang Anne Marie, Tsai Schickwann, Spangrude Gerald J
Department of Medicine, University of Utah School of Medicine, Salt Lake City 84132, USA.
Blood. 2004 Apr 15;103(8):2990-6. doi: 10.1182/blood-2003-09-3030. Epub 2003 Dec 30.
The recent description of an early T-lineage progenitor (ETP) population in adult mouse thymus implies the presence of a bone marrow predecessor that has not yet been identified. Here we describe a Lin(Neg) Sca-1(Pos) c-kit(Hi) Thy-1.1(Neg) L-selectin(Pos) adult mouse bone marrow population that resembles the thymic ETP in both antigen expression phenotype and posttransplantation lineage potential. These cells produce wavelike kinetics of thymic seeding and reconstitute the irradiated thymus with kinetics comparable to a thymocyte graft after intravenous transplantation. Transient B-lineage reconstitution is also observed, but little myeloid potential can be detected in transplant experiments. A second subset of progenitors is L-selectin(Neg) and is highly enriched for rapid and persistent T- and B-lineage potential, as well as some myeloid potential. L-selectin (CD62L) is therefore an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in mouse bone marrow.
最近在成年小鼠胸腺中发现了早期T细胞谱系祖细胞（ETP）群体，这意味着存在一种尚未被鉴定的骨髓前体细胞。在此，我们描述了一种成年小鼠骨髓群体，其Lin（阴性）Sca-1（阳性）c-kit（高表达）Thy-1.1（阴性）L-选择素（阳性），在抗原表达表型和移植后谱系潜能方面均类似于胸腺ETP。这些细胞产生波浪状的胸腺定植动力学，并在静脉内移植后以与胸腺细胞移植相当的动力学重建受照射的胸腺。还观察到短暂的B细胞谱系重建，但在移植实验中几乎检测不到髓系潜能。第二种祖细胞亚群是L-选择素（阴性），高度富集了快速且持久的T细胞和B细胞谱系潜能以及一些髓系潜能。因此，L-选择素（CD62L）是从小鼠骨髓中的髓系祖细胞和造血干细胞中分离淋巴系祖细胞的有效标志物。
