Manesh Saman K, Darling Cynthia L, Fried Daniel
Department of Preventative and Restorative Dental Sciences, University of California, San Francisco School of Dentistry, CA 94143-0758.
Proc SPIE Int Soc Opt Eng. 2009 Jan 1;7162. doi: 10.1117/12.816865.
Previous studies have demonstrated that polarization sensitive optical coherence tomography (PS-OCT) can be used to image natural root caries lesions, measure non-destructively the severity of dentin demineralization and determine the efficacy of intervention with anti-caries agents including fluoride and lasers. The objective of this study was to determine if PS-OCT could be used to nondestructively measure the formation of a layer of remineralized dentin on the surface of dentin lesions after exposure to a remineralization solution. In this study images of artificial dentin lesions on extracted human teeth were acquired using PS-OCT after exposure to an artificial demineralizing solution at pH 4.9 for six days and after subsequent exposure to a remineralizing solution at pH 7.0 for 20 days. Polarized light microscopy and microradiography were used to examine histological thin sections from the samples for comparison. PS-OCT successfully measured the formation of a layer of increased mineral content near the lesion surface. PLM and TMR corroborated those results. This study demonstrates the potential use of PS-OCT for the nondestructive measurement of the remineralization of dentin surfaces.
先前的研究表明，偏振敏感光学相干断层扫描（PS-OCT）可用于对天然根龋病变进行成像，无损测量牙本质脱矿的严重程度，并确定包括氟化物和激光在内的抗龋剂的干预效果。本研究的目的是确定PS-OCT是否可用于无损测量牙本质病变表面在暴露于再矿化溶液后再矿化牙本质层的形成情况。在本研究中，将拔除的人牙上的人工牙本质病变在pH 4.9的人工脱矿溶液中暴露6天，随后在pH 7.0的再矿化溶液中暴露20天，然后使用PS-OCT获取图像。使用偏振光显微镜和显微放射ography检查样本的组织学薄片以作比较。PS-OCT成功测量了病变表面附近矿物质含量增加层的形成。PLM和TMR证实了这些结果。本研究证明了PS-OCT在无损测量牙本质表面再矿化方面的潜在用途。
