Manesh Saman K, Darling Cynthia L, Fried Daniel
Department of Preventative and Restorative Dental Sciences, University of California, San Francisco School of Dentistry, CA 94143-0758.
Proc SPIE Int Soc Opt Eng. 2008 Jan 1;6843. doi: 10.1117/12.778788.
The purpose of this study was to assess the potential of polarization sensitive optical coherence tomography (PS-OCT) to measure non-destructively the severity of natural and artificial caries lesions in dentin and determine the efficacy of intervention with anti-caries agents. Although several studies have demonstrated the utility of PS-OCT to image caries lesions in enamel and to quantify the lesion severity, only a few studies have focused on lesions in dentin. In this study images of natural root caries lesions on extracted human teeth were acquired with PS-OCT. In addition artificial lesions were produced in dentin after 12 days of exposure to a demineralization solution at a pH of 5.0. Before exposure, three incisions were made on the sample surfaces using Er:YAG laser irradiation and selected areas were treated with topical fluoride. PS-OCT images were acquired using a high power (45-mw) 1310-nm superluminescent diode with a bandwidth of 35-nm. PS-OCT was able to measure demineralization in dentin to a depth of ~ 1 mm. Polarized light microscopy and microradiography were used to measure lesion severity on histological thin sections for comparison. PS-OCT successfully measured the inhibition of demineralization by topical fluoride. Er:YAG laser irradiation did not significantly increase or decrease the rate of dentin demineralization.
本研究的目的是评估偏振敏感光学相干断层扫描（PS-OCT）在无损测量牙本质中自然和人工龋损严重程度以及确定抗龋剂干预效果方面的潜力。尽管多项研究已证明PS-OCT在对釉质龋损进行成像和量化病变严重程度方面的效用，但仅有少数研究关注牙本质中的病变。在本研究中，使用PS-OCT获取了拔除的人牙上自然根龋病变的图像。此外，在pH值为5.0的脱矿溶液中暴露12天后，在牙本质中制造了人工病变。在暴露前，使用铒钇铝石榴石（Er:YAG）激光照射在样品表面制作三个切口，并对选定区域进行局部氟化物处理。使用功率为45毫瓦、带宽为35纳米的1310纳米高功率超发光二极管获取PS-OCT图像。PS-OCT能够测量牙本质中深度约为1毫米的脱矿情况。使用偏振光显微镜和显微放射ography测量组织学薄片上的病变严重程度以进行比较。PS-OCT成功测量了局部氟化物对脱矿的抑制作用。Er:YAG激光照射并未显著增加或降低牙本质脱矿速率。
