[2008年至2010年河南省分离的肠道病毒71型毒株的基因组特征与重组]

[Genomic characteristics and recombination of enterovirus 71 strains isolated in Henan Province between 2008 and 2010].

作者信息

Wei Hai-Yan, Xu Yu-Ling, Huang Xue-Yong, Ma Hong, Chen Hao-Min, Xu Bian-Li

机构信息

Henan Center for Disease Prevention and Control, Zhengzhou 450016, China.

出版信息

Bing Du Xue Bao. 2011 Sep;27(5):433-7.

PMID:21998954

Abstract

To reveal the genetic features and recombination of enterovirus 71 isolates between 2008 and 2010. A total of 5 enterovirus 71 isolates were sequenced completely and phylogenetic analysis and recombination were performed. Phylogenetic analysis based on VP1 regions revealed that the Henan enterovirus 71 between 2008 and 2010 belonged to C4a in subgenotype C4. Bootscan analyses and phylogenetic analysis based on the 5'UTR, P1, P2, P3 genomic regions revealed the recombinations between EV71 genotypes B and C at the 2A-2B junction, and between EV71 genotype B and CA16 strain G-10 at the 3B-3C junction. Henan enterovirus 71 isolates between 2008 and 2010 belonged to C4a in subgenotype C4 which was the predominant virus genotype circulating in mainland China since 2004, a combination of intratypic and intertypic recombination were found in EV71 subgenotype C4.

摘要

为揭示2008年至2010年间肠道病毒71型分离株的基因特征及重组情况。对5株肠道病毒71型分离株进行全基因组测序，并进行系统发育分析和重组分析。基于VP1区域的系统发育分析显示，2008年至2010年间的河南肠道病毒71型属于C4亚型中的C4a。基于5'UTR、P1、P2、P3基因组区域的Bootscan分析和系统发育分析显示，在2A-2B交界处存在肠道病毒71型B和C基因型之间的重组，在3B-3C交界处存在肠道病毒71型B基因型和CA16病毒株G-10之间的重组。2008年至2010年间的河南肠道病毒71型分离株属于C4亚型中的C4a，C4亚型是自2004年以来在中国内地流行的主要病毒基因型，在肠道病毒71型C4亚型中发现了型内重组和型间重组。