Huang Shih-Chen, Hsu Yun-Wei, Wang Hsuan-Chen, Huang Sheng-Wen, Kiang David, Tsai Huey-Pin, Wang Shih-Ming, Liu Ching-Chuan, Lin Kui-Hsiang, Su Ih-Jen, Wang Jen-Ren
Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Virus Res. 2008 Feb;131(2):250-9. doi: 10.1016/j.virusres.2007.10.002. Epub 2007 Nov 26.
Genetic recombination is a well-known phenomenon for enteroviruses. In this study, we determined the phylogenetic relationships of five distinct regions of the EV71 genome for 73 EV71 isolates from 1986 and from 1998 to 2005 in Taiwan. Phylogenetic analyses showed that the 5'-UTR, VP4-VP2, VP1, and 3D regions of EV71 isolated in 2004 and 2005 were grouped into genotype C. However, the 2B region of these isolates differed in that it grouped with genotype B, indicating recombination within EV71 had occurred. This intratypic recombination was first seen in 2002 and became predominant in 2004 and 2005. The simplot and bootscan analyses identified two recombination points located at the 3'-termini of the 2A and 3C regions. This intratypic recombination was identified among naturally circulating EV71 isolates in Taiwan, therefore, it suggests that nonstructural genes may recombine to produce new EV71 variants.
基因重组是肠道病毒中一种广为人知的现象。在本研究中，我们确定了来自台湾1986年以及1998年至2005年的73株EV71分离株的EV71基因组五个不同区域的系统发育关系。系统发育分析表明，2004年和2005年分离的EV71的5'-UTR、VP4-VP2、VP1和3D区域被归类为C基因型。然而，这些分离株的2B区域有所不同，它与B基因型归为一组，表明EV71内部发生了重组。这种型内重组首次出现在2002年，并在2004年和2005年变得 predominant。Simplot和bootscan分析确定了两个重组点，分别位于2A和3C区域的3'-末端。这种型内重组是在台湾自然传播的EV71分离株中发现的，因此，这表明非结构基因可能会重组产生新的EV71变体。 （注：“predominant”原文拼写有误，可能是“predominant”，意为“占主导地位的” ）
