He Ya-Qing, Qi Rui, Yang Hong, Xian Hui-Xia, Yao Xiang-Jie, Yang Fan, Zhang Hai-Long, Yang Xiao-Ke, Xu Wen-Bo
Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
Bing Du Xue Bao. 2009 Nov;25(6):415-9.
Genetic characteristics of enterovirus type 71 in Shenzhen from 2005 to 2008 were analyzed. All samples were detected by RT-PCR using EV71-specific primers. The VP1s of EV71 strains were amplified and sequenced. A phylogenetic tree was constructed by comparison of the sequences with those of subgenotype A, B and C using DNASTAR, BioEdit and Mega 3.1 softwares. The VP1 nucleotide sequences of 17 strains of Shenzhen shared 95.3%-99.4% identities with cluster C4a, and one strain shared 93.0%-95.6% identities with cluster C4b. The homogeneity of the VP1 nucleotide sequence with subgenotype A and B was 81.8%-86.0%. Among 18 strains, the homogeneity of the VP1 nucleotide sequence was between 92.5%-100%. All EV71 strains circulating in Shenzhen were of subgenotype C4. The predominant strain of EV71 belonged to cluster C4a, also there was cluster C4b.
分析了2005年至2008年深圳肠道病毒71型的基因特征。所有样本均使用肠道病毒71型特异性引物通过逆转录聚合酶链反应（RT-PCR）进行检测。扩增肠道病毒71型毒株的VP1并进行测序。使用DNASTAR、BioEdit和Mega 3.1软件，通过将序列与A、B和C亚基因型的序列进行比较构建系统发育树。深圳的17株毒株的VP1核苷酸序列与C4a簇的同源性为95.3%-99.4%，1株与C4b簇的同源性为93.0%-95.6%。与A和B亚基因型的VP1核苷酸序列同源性为81.8%-86.0%。在18株毒株中，VP1核苷酸序列同源性在92.5%-100%之间。深圳流行的所有肠道病毒71型毒株均为C4亚基因型。肠道病毒71型的优势毒株属于C4a簇，也有C4b簇。
