Pala Alessandro, D'Elia Piera, Spampinato Giuseppe, Pittaluga Eugenia, Benagiano Giuseppe
Department of Gynecological-Obstetric Sciences and Urological Sciences, Sapienza University of Rome, Rome, Italy.
J Matern Fetal Neonatal Med. 2012 Aug;25(8):1514-6. doi: 10.3109/14767058.2011.629258. Epub 2011 Nov 4.
Assessment of the capacity of Glycodelin A (GdA) to modulate the aggregation of cultured human umbilical vein endothelial cells.
Highly purified Glycodelin A (GdA) from late first trimester amniotic fluid has been added to cultured cells and its biological activity has been observed with immunofluorescent staining of β-catenin molecules.
GdA induces translocation of β-catenin molecules promoting cell-to-cell adhesion and formation of adherents junctions through cytoskeletal reorganization.
These data provide further mechanistic insight into the specificity of cell-to-cell adhesion, thus corroborating the role of GdA in promoting angiogenesis.
评估糖蛋白A（GdA）调节培养的人脐静脉内皮细胞聚集的能力。
将来自孕早期晚期羊水的高度纯化的糖蛋白A（GdA）添加到培养的细胞中，并通过β-连环蛋白分子的免疫荧光染色观察其生物活性。
GdA诱导β-连环蛋白分子易位，通过细胞骨架重组促进细胞间粘附和粘附连接的形成。
这些数据为细胞间粘附的特异性提供了进一步的机制性见解，从而证实了GdA在促进血管生成中的作用。
