Kim J G, Ramachandran S, Zhou H M, Rayner D, Parthasarathy S
Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, Georgia, USA.
Fertil Steril. 2000 Apr;73(4):839-42. doi: 10.1016/s0015-0282(99)00599-3.
We previously reported an antipeptide antibody to human glycodelin that recognizes glycodelin in amniotic fluid and epithelial glands of the endometrium. The objective of this study was to determine the presence of glycodelin in human umbilical cord.
Controlled clinical study.
Healthy women undergoing normal delivery at Grady Memorial Hospital, Atlanta, Georgia.
PATIENT(S): Healthy women undergoing normal delivery.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Umbilical cord vein was isolated from the cord and used for immunohistochemical studies. Human umbilical cords and placentae were collected after full-term delivery. Cords were washed and fixed with formal sucrose. Decidua tissues and blood vessels from placentae were dissected out and fixed. Cryostat sections were immunostained with anti-glycodelin peptide antibody and anti-von Willebrand factor antibody.
RESULT(S): Endothelial cells of human umbilical cord vein and artery were strongly immunostained with antiglycodelin antibody. Endothelial cells of the vein were more strongly stained than those of the artery. These cells were confirmed as endothelial cells by positive immunostaining with anti-von Willebrand factor. The epithelial cells outlining the cord were stained with antiglycodelin antibody but not with anti-von Willebrand factor antibody.
CONCLUSION(S): This is the first study showing that immunoreactive glycodelin is present in endothelial cells of the umbilical cord. Glycodelin in the umbilical cord may have immunosuppressive or other, unknown functions affecting the physiology or pathophysiology of pregnancy. Whether umbilical vein endothelial cells synthesize glycodelin or serve as reservoir for glycodelin is currently under investigation.
我们之前报道了一种针对人胎盘蛋白14的抗肽抗体，该抗体可识别羊水和子宫内膜上皮腺中的胎盘蛋白14。本研究的目的是确定人脐带中是否存在胎盘蛋白14。
对照临床研究。
佐治亚州亚特兰大市格雷迪纪念医院，正常分娩的健康女性。
正常分娩的健康女性。
无。
从脐带中分离出脐静脉用于免疫组织化学研究。足月分娩后收集人脐带和胎盘。脐带经冲洗后用甲醛蔗糖固定。从胎盘中解剖出蜕膜组织和血管并固定。冰冻切片用抗胎盘蛋白14肽抗体和抗血管性血友病因子抗体进行免疫染色。
人脐静脉和动脉的内皮细胞被抗胎盘蛋白14抗体强烈免疫染色。静脉内皮细胞的染色比动脉内皮细胞更强。通过抗血管性血友病因子的阳性免疫染色证实这些细胞为内皮细胞。勾勒脐带的上皮细胞被抗胎盘蛋白14抗体染色，但未被抗血管性血友病因子抗体染色。
这是第一项表明脐带内皮细胞中存在免疫反应性胎盘蛋白14的研究。脐带中的胎盘蛋白14可能具有免疫抑制或其他未知功能，影响妊娠的生理或病理生理。目前正在研究脐静脉内皮细胞是合成胎盘蛋白14还是作为胎盘蛋白14的储存库。
