Prrp K150 的乙酰化调节其亚细胞定位。

Acetylation of Prrp K150 regulates the subcellular localization.

机构信息

Graduate School of Environment and Information Science, Yokohama National University, Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan.

出版信息

Gene. 2012 Jan 1;491(1):13-9. doi: 10.1016/j.gene.2011.09.022. Epub 2011 Oct 6.

DOI:10.1016/j.gene.2011.09.022

PMID:22001406

Abstract

Posttranslational modifications of proteins have profound effects on many aspects of their function and have received much attention due to the importance of these processes in epigenetic regulation. In this study, we report that deleted azoospermia associated protein 1 (DAZAP1)/proline-rich RNA binding protein (Prrp), a multifunctional RNA binding protein which is essential for spermatogenesis and normal cell growth, is acetylated at Lysine 150 within its RNA binding domain. The acetylation is predominantly observed in nuclear Prrp, and the nonacetylated form is in cytoplasm. Considering that Prrp is a shuttling protein, we suggest that the acetylation cycle at Prrp K150 regulates nucleocytoplasmic transport in cells.

摘要

蛋白质的翻译后修饰对其功能的许多方面都有深远的影响，由于这些过程在表观遗传调控中的重要性，因此受到了广泛关注。在这项研究中，我们报告了缺失的无精子症相关蛋白 1（DAZAP1）/富含脯氨酸的 RNA 结合蛋白（Prrp），一种对精子发生和正常细胞生长至关重要的多功能 RNA 结合蛋白，其在 RNA 结合域的赖氨酸 150 位被乙酰化。乙酰化主要发生在核内 Prrp 中，而非乙酰化形式存在于细胞质中。考虑到 Prrp 是一种穿梭蛋白，我们推测 Prrp K150 上的乙酰化循环调节细胞的核质转运。