Department of Anesthesiology, the First Affiliated Hospital of Soochow University, Suzhou, 215006, PR China.
Pharmacol Rep. 2010 Jul-Aug;62(4):621-6. doi: 10.1016/s1734-1140(10)70319-4.
In the present study, we investigated the role of 5-hydroxytryptamine type 3 (5-HT(3)) receptors in hypnosis and analgesia induced by emulsified sevoflurane. A mouse model of hypnosis and analgesia was established by an intraperitoneal or subcutaneous injection of emulsified sevoflurane.We intracerebroventricularly (i.c.v.) or intrathecally (i.t.) administered YM-31636, a 5-HT(3) receptor agonist, to mice and observed sleep time during hypnosis. In addition, the tail withdrawal latency was measured using the tail withdrawal test, and the writhing time was determined using the acetic acid writhing test. In the hypnosis test, YM-31636 (5, 10 and 15 μg, i.c.v.) treatment significantly decreased emulsified sevoflurane-induced mouse sleep time (p < 0.05 or p < 0.01). YM-31636 (2.5, 5 and 10 μg, i.t.) treatment significantly and dose-dependently decreased the tail withdrawal latency (p < 0.05 or p < 0.01) and increased the writhing time (p < 0.01) of mice treated with emulsified sevoflurane. These results suggest that 5-HT(3) receptors may modulate the hypnotic and analgesic effects induced by emulsified sevoflurane.
在本研究中，我们研究了 5-羟色胺 3 型（5-HT3）受体在乳化七氟醚诱导的催眠和镇痛中的作用。通过腹腔或皮下注射乳化七氟醚建立了催眠和镇痛的小鼠模型。我们通过脑室内（i.c.v.）或鞘内（i.t.）给予 5-HT3 受体激动剂 YM-31636，观察催眠期间的睡眠时间。此外，使用尾部退缩测试测量尾部退缩潜伏期，使用醋酸扭体测试确定扭体时间。在催眠测试中，YM-31636（5、10 和 15μg，i.c.v.）处理显著减少了乳化七氟醚诱导的小鼠睡眠时间（p<0.05 或 p<0.01）。YM-31636（2.5、5 和 10μg，i.t.）处理显著且剂量依赖性地减少了尾部退缩潜伏期（p<0.05 或 p<0.01），并增加了乳化七氟醚处理的小鼠的扭体时间（p<0.01）。这些结果表明 5-HT3 受体可能调节乳化七氟醚诱导的催眠和镇痛作用。
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