Rodríguez-Ramírez R, Arana A, Alfonso L, González-Córdova A F, Torrescano G, Guerrero Legarreta I, Vallejo-Cordoba B
Laboratorio de Calidad, Autenticidad y Trazabilidad de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Hermosillo Sonora, México.
Genet Mol Res. 2011 Oct 6;10(4):2358-65. doi: 10.4238/2011.October.6.1.
Traceability ensures a link between carcass, quarters or cuts of beef and the individual animal or the group of animals from which they are derived. Meat traceability is an essential tool for successful identification and recall of contaminated products from the market during a food crisis. Meat traceability is also extremely important for protection and value enhancement of good-quality brands. Molecular meat traceability would allow verification of conventional methods used for beef tracing in synthetic Mexican bovine breeds. We evaluated a set of 11 microsatellites for their ability to identify animals belonging to these synthetic breeds, Brangus and Charolais/Brahman (78 animals). Seven microsatellite markers allowed sample discrimination with a match probability, defined as the probability of finding two individuals sharing by chance the same genotypic profile, of 10(-8). The practical application of the marker set was evaluated by testing eight samples from carcasses and pieces of meat at the slaughterhouse and at the point of sale. The DNA profiles of the two samples obtained at these two different points in the production-commercialization chain always proved that they came from the same animal.
可追溯性确保了牛肉胴体、四分体或切块与它们所来自的单个动物或动物群体之间的联系。肉类可追溯性是在食品危机期间成功识别和召回市场上受污染产品的一项重要工具。肉类可追溯性对于优质品牌的保护和价值提升也极为重要。分子肉类可追溯性将能够验证墨西哥合成牛品种中用于牛肉溯源的传统方法。我们评估了一组11个微卫星，以确定它们识别属于这些合成品种（婆罗门牛和夏洛莱/婆罗门牛，共78头动物）的动物的能力。七个微卫星标记能够进行样本区分，其匹配概率（定义为偶然发现两个个体具有相同基因型的概率）为10^(-8)。通过对屠宰场和销售点的八个胴体和肉块样本进行检测，评估了该标记集的实际应用。在生产-商业化链中这两个不同点获得的两个样本的DNA图谱始终证明它们来自同一动物。
