Orrú L, Napolitano F, Catillo G, Moioli B
Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy.
Meat Sci. 2006 Feb;72(2):312-7. doi: 10.1016/j.meatsci.2005.07.018. Epub 2005 Oct 3.
To set up routine assays for molecular meat traceability along the food chain, the availability of a simple and low cost test for the identification of cattle carcasses is required. For this purpose, we evaluated 13 microsatellites for their ability in the identification of animals belonging to four Italian cattle breeds. Here we propose a criterion for a microsatellite-based test with the best reliability when reducing the number of loci to be analysed. The method is based on the observation that in the same loci breeds can show differences in frequencies and number of fixed alleles. This non-uniform distribution of alleles between breeds results in differences in the informative content of the same loci in different breeds. Taking into account these differences, it is possible to perform tests for the allocation of samples to specific animals utilizing a small number of microsatellites. The proposed approach allows cost reduction and ease in performing the analyses.
为了建立沿食物链的分子肉类可追溯性常规检测方法，需要一种简单且低成本的用于识别牛胴体的检测方法。为此，我们评估了13个微卫星标记识别四个意大利牛品种动物的能力。在此，我们提出一种基于微卫星的检测标准，在减少待分析基因座数量时具有最佳可靠性。该方法基于这样的观察结果：在相同基因座上，不同品种的等位基因频率和固定等位基因数量可能存在差异。品种间等位基因的这种非均匀分布导致相同基因座在不同品种中的信息含量不同。考虑到这些差异，利用少量微卫星标记就可以对样本进行特定动物的分配检测。所提出的方法能够降低成本并简化分析过程。
