比较四种方法评估小鼠附睾头和尾精子 DNA 完整性。
Comparison of four methods to evaluate sperm DNA integrity between mouse caput and cauda epididymidis.
机构信息
Department of Animal Reproduction, National Institute for Agricultural and Food Research and Technology, Madrid 28040, Spain.
出版信息
Asian J Androl. 2012 Mar;14(2):335-7. doi: 10.1038/aja.2011.119. Epub 2011 Oct 17.
Abstract
It is well known that transit through the epididymis involves an increase in the compaction of sperm chromatin, which acquires fully condensed status at the caput epididymidis. The purpose of this study was to compare the terminal deoxyribonucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) assay, the comet assay, the sperm chromatin structure assay (SCSA) and the sperm chromatin dispersion (SCD) test by analysing spermatozoa from the caput and cauda epididymidis in order to demonstrate the ability of each technique to discriminate between different degrees of sperm maturity related to chromatin compaction and DNA fragmentation. Our results suggest that some populations of DNA-fragmented spermatozoa associated with immature sperm can only be identified using the comet assay and the SCSA but not with the SCD test or the TUNEL assay.
摘要
众所周知，精子通过附睾时，其染色质会发生浓缩，在附睾头部达到完全浓缩状态。本研究旨在通过比较末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记（TUNEL）检测、彗星试验、精子染色质结构分析（SCSA）和精子染色质扩散（SCD）试验，分析附睾头和附睾尾精子，以证明每种技术区分与染色质浓缩和 DNA 碎片化相关的不同成熟程度精子的能力。我们的结果表明，一些与不成熟精子相关的 DNA 碎片化精子群体只能通过彗星试验和 SCSA 检测识别，而不能通过 SCD 试验或 TUNEL 检测识别。
相似文献
1
Comparison of four methods to evaluate sperm DNA integrity between mouse caput and cauda epididymidis.
Asian J Androl. 2012 Mar;14(2):335-7. doi: 10.1038/aja.2011.119. Epub 2011 Oct 17.
2
Measurement of sperm DNA fragmentation using bright-field microscopy: comparison between sperm chromatin dispersion test and terminal uridine nick-end labeling assay.
Fertil Steril. 2010 Aug;94(3):1027-32. doi: 10.1016/j.fertnstert.2009.04.034. Epub 2009 Jun 7.
3
Effects of caput ligation on rat sperm and epididymis: protein thiols and fertilizing ability.
Biol Reprod. 1992 Feb;46(2):301-8. doi: 10.1095/biolreprod46.2.301.
4
The Sperm Chromatin Structure Assay (SCSA(®)) and other sperm DNA fragmentation tests for evaluation of sperm nuclear DNA integrity as related to fertility.
Anim Reprod Sci. 2016 Jun;169:56-75. doi: 10.1016/j.anireprosci.2016.01.017. Epub 2016 Feb 17.
5
Comparison of DNA fragmentation of frozen-thawed epididymal sperm of dogs using Sperm Chromatin Structure Analysis and Sperm Chromatin Dispersion test.
Anim Reprod Sci. 2017 Dec;187:74-78. doi: 10.1016/j.anireprosci.2017.10.007. Epub 2017 Oct 12.
6
Comparison of chromatin assays for DNA fragmentation evaluation in human sperm.
J Androl. 2006 Jan-Feb;27(1):53-9. doi: 10.2164/jandrol.05068.
7
Measuring Sperm DNA Fragmentation and Clinical Outcomes of Medically Assisted Reproduction: A Systematic Review and Meta-Analysis.
PLoS One. 2016 Nov 10;11(11):e0165125. doi: 10.1371/journal.pone.0165125. eCollection 2016.
8
Validation of the sperm chromatin dispersion (SCD) test in the amphibian Xenopus laevis using in situ nick translation and comet assay.
Reprod Fertil Dev. 2015 Nov;27(8):1168-74. doi: 10.1071/RD14070.
9
[Sperm DNA integrity as diagnosis and prognosis element of male fertility].
Gynecol Obstet Fertil. 2005 Mar;33(3):89-101. doi: 10.1016/j.gyobfe.2005.02.012.
10
Sperm Chromatin Dispersion Test Detects Sperm DNA Fragmentation Mainly Associated with Unviable Spermatozoa and Underestimates the Values with Respect to TUNEL Assay.
Int J Mol Sci. 2024 Apr 19;25(8):4481. doi: 10.3390/ijms25084481.
引用本文的文献
1
Sperm chromatin structure assay (SCSA) and flow cytometry-assisted TUNEL assay provide a concordant assessment of sperm DNA fragmentation as a function of age in a large cohort of approximately 10,000 patients.
Basic Clin Androl. 2023 Nov 30;33(1):33. doi: 10.1186/s12610-023-00208-9.
2
External quality assessment scheme for sperm DNA fragmentation: a pilot study in China.
Basic Clin Androl. 2023 Nov 28;33(1):36. doi: 10.1186/s12610-023-00211-0.
3
Sperm Chromatin Status and DNA Fragmentation in Mouse Species with Divergent Mating Systems.
Int J Mol Sci. 2023 Nov 3;24(21):15954. doi: 10.3390/ijms242115954.
4
Loss of the importin causes infertility in male mice by disrupting the translocation of testis-specific transcription factors.
iScience. 2023 Jun 16;26(7):107134. doi: 10.1016/j.isci.2023.107134. eCollection 2023 Jul 21.
5
Direct but Not Indirect Methods Correlate the Percentages of Sperm With Altered Chromatin to the Intensity of Chromatin Damage.
Front Vet Sci. 2021 Aug 25;8:719319. doi: 10.3389/fvets.2021.719319. eCollection 2021.
6
Comparing the different methods of sperm chromatin assessment concerning ART outcomes.
Turk J Urol. 2020 Sep;46(5):348-353. doi: 10.5152/tud.2020.19262. Epub 2020 May 27.
7
Successful ICSI in Mice Using Caput Epididymal Spermatozoa.
Front Cell Dev Biol. 2019 Dec 17;7:346. doi: 10.3389/fcell.2019.00346. eCollection 2019.
8
Sperm DNA and detection of DNA fragmentations in sperm.
Turk J Urol. 2018 Jan;44(1):1-5. doi: 10.5152/tud.2018.49321. Epub 2018 Jan 8.
9
Sperm selection by thermotaxis improves ICSI outcome in mice.
Sci Rep. 2018 Feb 13;8(1):2902. doi: 10.1038/s41598-018-21335-8.
10
Microfluidics for sperm analysis and selection.
Nat Rev Urol. 2017 Dec;14(12):707-730. doi: 10.1038/nrurol.2017.175. Epub 2017 Oct 31.
本文引用的文献
1
TUNEL assay and SCSA determine different aspects of sperm DNA damage.
Andrologia. 2010 Oct;42(5):305-13. doi: 10.1111/j.1439-0272.2009.01002.x.
2
DNA status on thawed semen from fighting bull: a comparison between the SCD and the SCSA tests.
Reprod Domest Anim. 2009 Jun;44(3):424-31. doi: 10.1111/j.1439-0531.2008.01098.x. Epub 2008 Nov 4.
3
The comet assay: topical issues.
Mutagenesis. 2008 May;23(3):143-51. doi: 10.1093/mutage/gem051. Epub 2008 Feb 17.
4
Scrotal heat stress effects on sperm viability, sperm DNA integrity, and the offspring sex ratio in mice.
Mol Reprod Dev. 2008 Jan;75(1):40-7. doi: 10.1002/mrd.20759.
5
The comet assay: a method to measure DNA damage in individual cells.
Nat Protoc. 2006;1(1):23-9. doi: 10.1038/nprot.2006.5.
6
DNA fragmentation assessment by flow cytometry and Sperm-Bos-Halomax (bright-field microscopy and fluorescence microscopy) in bull sperm.
Int J Androl. 2007 Apr;30(2):88-98. doi: 10.1111/j.1365-2605.2006.00723.x. Epub 2006 Dec 7.
7
Comparison of chromatin assays for DNA fragmentation evaluation in human sperm.
J Androl. 2006 Jan-Feb;27(1):53-9. doi: 10.2164/jandrol.05068.
8
Plasma membrane structure and remodelling during sperm maturation in the epididymis.
J Reprod Fertil Suppl. 1998;53:73-84.
9
Changes in chromatin condensation of human spermatozoa during epididymal transit as determined by flow cytometry.
Hum Reprod. 1996 Jul;11(7):1457-62. doi: 10.1093/oxfordjournals.humrep.a019419.
10
Function of human epididymal spermatozoa.
J Androl. 1994 Nov-Dec;15 Suppl:23S-27S.
Zotero 插件