Haidl G, Badura B, Schill W B
Department of Dermatology and Andrology, Justus Liebig University, Giessen, Germany.
J Androl. 1994 Nov-Dec;15 Suppl:23S-27S.
Human epididymal spermatozoa taken from caput, corpus, and cauda were investigated to determine their fertilizing capacity (22 epididymides from 11 patients who had undergone orchidectomy because of prostatic cancer). The following functions, which have been reported to correlate positively with the fertilization rate, were determined: motility and progressive motility, chromatin condensation (assessed by aniline blue staining), acrosin activity, and induction of acrosome reaction by low temperature. In addition, stimulation of motility by pentoxifylline and phosphatidylcholine was examined. The results showed that motility, progressive motility, normal chromatin condensation, and inducible acrosome reaction increased from the caput to the cauda epididymidis, whereas acrosin activity was normal in all sections. Stimulation of progressive motility, especially that of caput spermatozoa, could be achieved by both pentoxifylline and phosphatidylcholine, the latter being definitely superior. In conclusion, our study confirmed that human spermatozoa in physiological status undergo several steps of maturation during the epididymal transit. Stimulation of sperm motility by phosphatidylcholine may be helpful for patients in whom epididymal spermatozoa are used for assisted reproduction.
对取自附睾头、附睾体和附睾尾的人类附睾精子进行研究，以确定其受精能力（取自11例因前列腺癌接受睾丸切除术患者的22个附睾）。测定了以下据报道与受精率呈正相关的功能：活力和前向运动能力、染色质凝聚（通过苯胺蓝染色评估）、顶体蛋白酶活性以及低温诱导顶体反应。此外，还检测了己酮可可碱和磷脂酰胆碱对精子活力的刺激作用。结果显示，活力、前向运动能力、正常染色质凝聚和可诱导的顶体反应从附睾头到附睾尾逐渐增加，而顶体蛋白酶活性在所有部位均正常。己酮可可碱和磷脂酰胆碱均可刺激前向运动能力，尤其是附睾头精子的前向运动能力，其中磷脂酰胆碱明显更具优势。总之，我们的研究证实，处于生理状态的人类精子在附睾转运过程中经历了几个成熟步骤。磷脂酰胆碱对精子活力的刺激可能有助于使用附睾精子进行辅助生殖的患者。
