Aix-Marseille Univ., IRD, MIO UMR 235; CNRS/INSU, MIO UMR 7294, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
Laboratoire des Bio-Procédés Environnementaux, Centre de Biotechnologie de Sfax, BP 1177, 3018 Sfax, Tunisia.
Int J Syst Evol Microbiol. 2012 Aug;62(Pt 8):1970-1973. doi: 10.1099/ijs.0.036301-0. Epub 2011 Oct 14.
An obligatory anaerobic, moderately halophilic bacterium, designated strain CEJFG43(T), was isolated from a sample of sediment collected below the salt crust on the hypersaline El Jerid lake, in southern Tunisia. The cells of this novel strain were Gram-staining-negative, non-sporulating, motile, short rods. They grew in media with 6-30% (w/v) NaCl (optimum 15%), at 20-60 °C (optimum 45 °C) and at pH 5.5-9.5 (optimum pH 8.3). The micro-organism fermented glucose, fructose, ribose, raffinose, galactose, mannose, sucrose, maltose, xylose, mannitol, pyruvate and glycerol. The products of glucose fermentation were lactate, ethanol, acetate, H(2) and CO(2). The genomic G+C DNA content of strain CEJFG43(T) was 33.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CEJFG43(T) belonged in the genus Halanaerobacter and was most closely related to Halanaerobacter lacunarum DSM 6640(T) (95.3% gene sequence similarity) and Halanaerobacter chitinivorans DSM 9569(T) (95.3%). The predominant cellular fatty acids were non-branched (C(16:0) and C(16:1)). Based on the phylogenetic and phenotypic evidence, strain CEJFG43(T) represents a novel species in the genus Halanaerobacter for which the name Halanaerobacter jeridensis sp. nov. is proposed. The type strain is CEJFG43(T) ( = DSM 23230(T) = JCM 16696(T)).
一株严格的厌氧菌，中度嗜盐菌，命名为 CEJFG43(T) 菌株，从突尼斯南部的杰里德盐湖盐壳下采集的沉积物样本中分离得到。该新菌株的细胞革兰氏染色阴性，不产芽孢，运动，短杆状。它们在含有 6-30%（w/v）NaCl（最佳 15%）的培养基中生长，在 20-60°C（最佳 45°C）和 pH5.5-9.5（最佳 pH8.3）下生长。微生物发酵葡萄糖、果糖、核糖、棉子糖、半乳糖、甘露糖、蔗糖、麦芽糖、木糖、甘露醇、丙酮酸和甘油。葡萄糖发酵的产物为乳酸、乙醇、乙酸、H(2)和 CO(2)。CEJFG43(T) 菌株的基因组 G+C DNA 含量为 33.3 mol%。基于 16S rRNA 基因序列的系统发育分析表明，CEJFG43(T) 菌株属于盐厌氧菌属，与 Halanaerobacter lacunarum DSM 6640(T)（95.3%的基因序列相似性）和 Halanaerobacter chitinivorans DSM 9569(T)（95.3%的基因序列相似性）最为密切相关。主要的细胞脂肪酸是非支链的（C(16:0) 和 C(16:1)）。基于系统发育和表型证据，CEJFG43(T) 菌株代表盐厌氧菌属中的一个新种，现提议将其命名为杰里德盐厌氧菌（Halanaerobacter jeridensis sp. nov.）。模式菌株为 CEJFG43(T)（=DSM 23230(T)=JCM 16696(T)）。
